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Questions related to Liquid Chromatography
I found that my HPLC fluorescence detector couldn’t quantify tocopherol 1000ppm while an UV detector can. Why does this happen? Is it that the fluorescence detector is too sensitive for higher...
10 February 2014 5,785 8 View
Gas chromatography is always used for the assay of muramic acid content in the cell. Could I use LC instead ?
06 February 2014 1,942 3 View
I am trying to do a research on the levels of MTBE in industrial and domestic wastewater. I need some assistance.
02 February 2014 6,612 8 View
I'm new to polyamide column and I don't know the technique.
02 February 2014 9,085 9 View
I'm trying to develop an HPLC method to determine MEROPENEM concentration in blood samples. A strange phenomenon has happened on preliminary testing to determine retention times of meropenem. I´m...
02 February 2014 3,177 16 View
Need some clear cut clarification. it may be useful for my upcoming works.
02 February 2014 3,428 2 View
02 February 2014 8,222 9 View
02 February 2014 2,643 3 View
I want to understand how this software works in a QTOF-MS instrument
02 February 2014 5,039 1 View
I have a "problem" with the export format files (random code). I would like that Empower export my files with the exact sample name and without this random code. For example: sample name= FCPG597;...
01 January 2014 677 0 View
Using very complex samples (blood plasma), I need to estimate how much quantity of raw sample can be injected into a nano / micro / narrow-bore column. Is there a "rule" related to column...
01 January 2014 7,920 9 View
I need to check some parameters of analysis (reagents, column, temperature...) to compare to the method used in my job (liquid chromatography)
01 January 2014 1,494 0 View
No liquid PITC available.
01 January 2014 5,342 2 View
I experienced, that when I used 3 or 5 kd cut-off filter tubes to exchange buffer for proteomics work, the flow through have some protein content. Theoretically it should not have happened. In...
01 January 2014 7,761 3 View
I have gotten the Vitamin C to stick to a C18 using methanol at 0.5 mL/min, but it always comes out in a blob when I run a food sample. I have set the UV to 243, 250 and 260 to try and phase out...
01 January 2014 8,756 26 View
I want to calculate the range of confidence interval in between which my data are lying. Suggests please.
01 January 2014 9,766 2 View
I'm using commercial Herceptin for some experiments and when running FPLC (column superdex 200, PBS mobile phase, eluting 0.5ml/min) on my samples I find, besides the antibody peak (UV 280nm) at...
29 November 2013 1,676 3 View
I have done analysis of LC-MS/MS using band of SDS PAGE result, I have got the data..but there are some data I don't yet understand how to read, ie : total, % cov, % cov (50) and % cov (95)....
15 November 2013 7,984 3 View
11 November 2013 1,787 4 View
Any clue on the reasons for low recovery % in drug tablets? I got 64% and 68% for PCM in a drug sample analyzed on RP-HPLC using ACN: 50 mM phosphate buffer (60:40) on a C-18 YMC HPLC column.
11 November 2013 9,672 1 View
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11 November 2013 518 7 View
11 November 2013 4,127 3 View
If I have the choice between 2 amino columns for carbohydrate analysis: Luna amino column from phenomenex and Pinnacle from restek which one should I choose?
11 November 2013 3,459 6 View
I have followed the journal that I refer but the solution I prepared to dissolve and dilute my standards was clear at the beginning. After 2 to 3 minutes it started to be cloudy. Can anyone can...
11 November 2013 1,639 14 View