Will you prefer taqman or sybrgreen RTPCR?

Have you seen any difference in Taqman system and Sybrgreen RTPCR? Former is way expensive?

11 December 2017 5,505 0 View

Is Predatory Journals Omnics publisher is obslete?

31 December 2016 1,640 0 View

How we can change climate?

The conference of climate change is underway- How demonstrations are helping them? Though it is each one's responsibility. Are these demonstrations political or real people?

10 November 2015 2,852 1 View

What you think about the punishment to Dr. Hunt on his comments of females?

Sir Tim Hunt, a nobel laureate was severely punished and deprived of his whole life hard work and career reputation for the comment he made, he claimed that he did a joke only. As human beings...

06 July 2015 4,171 3 View

Which is good working antibody for MMP13 specific for human and rat for immunohistochemistry?

If someone know good working antibody for MMP13 specific for human and rat, pl share the catalog number. I appreciate, Thanks, Subhash

06 July 2015 4,225 7 View

Anyone suggest me for free software for drawing chemical structures for MAC desktop?

This question is towards organic chemistry/molecular biology researchers. I am looking for basic chemical structures drawing free software for Mac preferably. You may suggest for PC also. Thanks...

01 February 2015 7,557 6 View

Do you check and respond office e-mails after hours?

01 February 2015 6,245 5 View

What is immediate reaction to negative results of a big experiment?

I like you to share what is your immediate reaction to negative results of a big experiment which are contrary to your expectations. How you deal with it.

31 December 2014 1,427 9 View

Why all journals don't follow one universal style of research publication?

Every journal has different rules of writing style, bibliography style, figures presentation, number of references, number of figures and number of tables, number of pages limit etc. All this is...

11 December 2014 4,934 13 View

Do you prefer blind review process of your research paper?

Some journals get their research reviewed after hiding the names of the authors. What is your preference and why? What is the fair review process: blind or open

10 November 2014 8,526 14 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View