Can you take 1 ml blood aliquots from your subjects? over what time frame would the drug be administered.  To check if acquired immunity is modulated use blood collected in heparinised vacuum tubes. Do the following under aseptic conditions:

Add 40 ul blood to 360 ul RPMI1640 culture medium (control)

Add 40 ul blood to 360 ul RPMI1640 containing 16 ug/ml phytohemagglutinin (stimulated sample).

Prepare each in triplicate.  Incubate overnight at 37 C in a 5 % CO2 incubator.

Collect the supernatants and screen for IFNg (cell mediated immunity) and IL10 (humoral immunity). This can be done using an ELISA or FACS. Most hospital pathology labs would assay these cytokines regularly.

Cytokine levels should be compared for blood taken before drug therapy and after drug therapy. Changes in these cytokine levels will tell you if the drug affects these two pathways.

The same can be done for inflammatory activity.  We use 1 ug/ml LPS as stimulant and IL6 as biomarker for this pathway.

There are several other markers that can also be used for these pathways.  I have just given you the once that we used previously for checking immune status.

Can you take 1 ml blood aliquots from your subjects? over what time frame would the drug be administered.  To check if acquired immunity is modulated use blood collected in heparinised vacuum tubes. Do the following under aseptic conditions:

Add 40 ul blood to 360 ul RPMI1640 culture medium (control)

Add 40 ul blood to 360 ul RPMI1640 containing 16 ug/ml phytohemagglutinin (stimulated sample).

Prepare each in triplicate.  Incubate overnight at 37 C in a 5 % CO2 incubator.

Collect the supernatants and screen for IFNg (cell mediated immunity) and IL10 (humoral immunity). This can be done using an ELISA or FACS. Most hospital pathology labs would assay these cytokines regularly.

Cytokine levels should be compared for blood taken before drug therapy and after drug therapy. Changes in these cytokine levels will tell you if the drug affects these two pathways.

The same can be done for inflammatory activity.  We use 1 ug/ml LPS as stimulant and IL6 as biomarker for this pathway.

There are several other markers that can also be used for these pathways.  I have just given you the once that we used previously for checking immune status.

Thank you Sir, that was really helpful.

Apart from the IFN Gamma  and cytokine studies as suggested by Edmund Pool You can measure the total serum immunoglobulins and IgG pre & post treatment

You can also test for phagocytic index/ capacity of neutrophils and macrophages

Any effect on The respiratory burst can be detected by NBT reduction test.

 There are also changes in the primary and secondary immune organs in B cell areas and T-cell areas which can be visualized after histopathology in case of experimental animals.

Thank you sir, that is an 'add-on' to the tests.

Would there be any publication that would detail methods used describe above. It will be greatly appreciated.