Is there a method to induce the proliferation of dendritic cells or CD14+ T cells
A hall mark of CD14 positive monocytic cells (or DC derived from them) is that they dont really proliferate anymore in culture. Monocytes, once left the bone-marrow are fully matured cells, that can be matured into macrophages (in addition to M-CSF, you could also use CSF1). If you work from blood derived cells, you can change the phenotype/function of monocytes into either macrophages OR dendritic cells. But there are definitively no CD14+ T cells.
ok did you try that by yourself , the point is that i want to be sure that the cells after the proliferation do not change their phenotype (if they are DCs or CD14 +ve cells)
We have used PDGF-BB (R&D Laboratories) in our cultures of adult-derived totipotent stem cells, pluripotent stem cells, and mesodermal stem cells. It will not induce differentiation in any of our stem cells through multiple passages. You will need to titrate amount based on your cell type. Other agents that have been described in the literature for inducing proliferation actually have multiple activities, proliferation being only one of them.
Hello
Yes , you can stimulate the proliferation T cells by CD3 and CD28 and CD14
see attachment , Good Luck .
Firstly, are u sure there are `CD14 T cells'? CD14 usually is used for identify human blood monocytes, and these cells can be induced into different macrophage in different condition. Basically, human M-CSF induce CD14+ cells into anti-inflammatory M2-like macrophage, human GM-CSF induce them into pro-inflammatory M1-like macrophage.To induce dendritic cells, we always use GM-CSF+IL-4 for mice bone marrow cells. Normally, if proliferation always come up with differentiation in these CD14 cells, so be careful and good luck!
A hall mark of CD14 positive monocytic cells (or DC derived from them) is that they dont really proliferate anymore in culture. Monocytes, once left the bone-marrow are fully matured cells, that can be matured into macrophages (in addition to M-CSF, you could also use CSF1). If you work from blood derived cells, you can change the phenotype/function of monocytes into either macrophages OR dendritic cells. But there are definitively no CD14+ T cells.
Monocyte derived DCs (from CD14+ blood monocytes) and monocytes themselves don't proliferate in cultures. Of course its different if you talk about T cells, but to my knowledge CD14 is not a marker of T cells.
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