Looking for high efficient light sources (Laser, HE LED) for Spectroscopic Ellipsometry?

Conventional light sources have a penetration depth of a few nanometers in metal layers and cannot be used for spectroscopic ellipsometry purpose to measure layer thicknesses of a few 100 nanometres.

27 October 2020 7,663 4 View

Why is fungal/bacterial DNA isolated with Qiagen Powerlyzer Powersoil Kit not stable?

We have isolated DNA with the Qiagen Powerlyzer Powersoil Kit (with addition of Proteinase K) for NGS downstream applications (16SrRNA/ITS2) and encountered the problem that the isolated DNA is...

26 October 2020 7,418 2 View

Which is more radiosensitive, fungal spores or the mycelium itself?

Is there any evidence suggesting that spores are more resistant to radiation than the mycelia?

07 May 2017 1,436 4 View

Can UV-C or any other commercially viable type of UV Ray penetrate tissue of agricultural products such as coconut meat or copra?

I have trouble searching for researches that uses UV treatment on agricultural products. It is mainly used for liquid disinfection so I'm curious if it can work on opaque organic products.

21 August 2016 8,327 3 View

How can I stain for dead neurons in the ischemic mouse brain?

I am looking for a staining method to label dead neurons in the ischemic mouse brain.I use coronar cryosection from mice which were subjected to transient MCAo and reperfusion. I tried NeuN+TUNEL...

09 June 2016 7,426 3 View

Is it possible to use desert sand as a building material?

Like mortar or concrete? I can not find case studies or articles about this topic?

15 May 2016 8,157 27 View

Is there recent knowledge about social structure within a litter of warthog piglets?

Young warthogs (Phacochoerus aethiopicus) display suspicious light colored bristels at their cheeks. In our view these seem to mimic the prominent tuskers of the adult...(?) I would greatly...

06 October 2015 1,983 4 View

Has anyone ever tried to detect Blue Jets, Elves and Sprites above Volcanic Eruption Clouds ?

Many years back, I attended a special session on Elves, Sprites and Blue Jets at AGU in San Francisco. These are typically observed above severe thunderstorm clouds and are observed at something...

27 August 2015 4,229 2 View

Is Qiagen AllPrep DNA/RNA FFPE kit compatible with Laser Capture Micro-dissection using arcturus capsure HS LCM caps?

08 March 2015 1,823 2 View

Is Indel Realignment recommended previous to Structural Variant Calling?

Is there any experience how GATK's IndelRealignment influences the results of subsequent Structural Variant Calling, e.g. with DELLY? Does Indel Realignment improve the predictions or should be...

27 November 2014 2,300 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View