Identification and quantification of senescent cells in human tissues is a challenging task. It is well known that the application of a single biomarker such as SA-b-gal activity (the most widely used senescent marker) is not enough. Today we can chose among a large variety of senescent markers, such as gammaH2AX, Ki67, BrdU, HGMB1, p16INK4A, p15INK4B, p21CIP1, p53, DEC1, DCR2, B2MG and PAI1.
What combination will be the most efficient and cost effective?
In a fresh tissue sample that contains mainly senescent mesenchymal cells, SA-beta-Gal staining plus staining for p21 CDKI would help determine senescent cells. In a fresh tissue with mainly non-mesenchymal cells, staining for SA-beta-gal plus p16 staining would be informative.
In a fresh tissue sample that contains mainly senescent mesenchymal cells, SA-beta-Gal staining plus staining for p21 CDKI would help determine senescent cells. In a fresh tissue with mainly non-mesenchymal cells, staining for SA-beta-gal plus p16 staining would be informative.
SenTraGor. This is quite novel stain to detect senescence, both in vitro and in vivo. It's advantage is the possibility to co stain another markers of senescence eg. p21 or p16.
Here is the link: http://sentragortechnology.com/
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