take the 1:1 mixture and throw it in bleach water.  you should get a bright flash of light.  

take the 1:1 mixture and throw it in bleach water.  you should get a bright flash of light.  

Load you 2nd antibody to the PVDF membrane, then incubate ECL reagent with this membrane after it is dry. 

Add a uL of your HRP-linked secondary antibody to a couple of mL of your prepared substrate. Take to the darkroom - it should glow under red light.