Dear colleagues,
I have problem with Fast Green FCF, I used this dye to stain cotyledons. I try 0,5% Fast Green in alcohol and methyl salicylate (1:1), and 0,1% Fast Green in 96% alkohol and always the colour is very strong and I see oil droplets. I used after staining clearing solution (methyle salicylate:xylene:EtOh).
Somebody has the same problem or knows how to avoid it
Dear Paulina, I am sorry, I can't help you practically, but:
have you found the page on
, including
JOHANSEN’S SAFRANIN AND FAST GREEN
[Johansen’s method (Johansen, 1940)] differs from most other Safranin/Fast Green protocols in the use of additions to the stain and clearing solutions to enhance and differentiate tissue structure.
you can find also:
SASS’S SAFRANIN AND FAST GREEN
This method of Sass (1958) uses fewer additions than Johansen’s method (above) but works adequately (NB: Some workers, [Refs] note, however, that aqueous or weak alcoholic solutions of Safranin O yield markedly inferior results compared to Johansen’s method.)
cf: @ http://microscopy.berkeley.edu/Resources/instruction/staining.htm
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