Cell culture
Biotechnology
What is the range of concentration and exposure time of PFA for fixing of mammalian cells with 96 well plate?
It is 4% PFA (pH 7.5). Cells are incubated for 10 min at room temperature or 20 min at 0 C.
Ideally people go for 2-4% PFA and fix for about 20-30 minutes.
Cheers
I have generally used 4% PFA for 15 minutes to fix endothelial cells either on coverslips or in 96 well plates
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