Samples are fixed, embedded and frozen following a standard protocol though when sectioned at 10 um the tissue is flimsy, delicate, and missing sections.
if it is between E8 and E11, I would recommend you to fix it in 4% PFA overnight at 4 degrees/cold room. Most of the protocols mention fixation upto a few hrs. Overnight treatment with PFA makes the tissue more stable. wash once with PBS and then leave it in 20-30% sucrose (made in PBS) overnight at 4 degrees. wash again with pbs and then Embed it in OCT using dry ice. the the whole embryo at -15 degrees in the cryostat...should work fine..let me know if theres something
if it is between E8 and E11, I would recommend you to fix it in 4% PFA overnight at 4 degrees/cold room. Most of the protocols mention fixation upto a few hrs. Overnight treatment with PFA makes the tissue more stable. wash once with PBS and then leave it in 20-30% sucrose (made in PBS) overnight at 4 degrees. wash again with pbs and then Embed it in OCT using dry ice. the the whole embryo at -15 degrees in the cryostat...should work fine..let me know if theres something