The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
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Questions related to Gene Expression
I am using siRNA knock down to evaluate changes in gene expression in cancer cells. I found that GAPD is an unsuitable reference gene for my system given that it highly variates between...
11 November 2019 2,298 5 View
I’m new to this kind of data set, and we received data back from a company on gene expression of various genes In the form of log2 values. I’m not sure how to calculate fold change from these...
11 November 2019 5,115 5 View
I want to observe differential gene expression of cells that have a knockout of this gene versus another type of cell that normally does not express this gene. Do you know where I can find the...
11 November 2019 6,149 1 View
If I want to use high expression of my target protein (due to its low activity) in E.coli in shake flask as well as pilot scale bioreactor ? What are the factors that I must bear in mind ? Low or...
11 November 2019 1,146 1 View
Hello, I am trying to determine if a region present in a Streptomyces genome acts as a ncRNA that affects gene expression. Which are the best tools to analyze sequences for this? Thanks!
11 November 2019 3,287 2 View
Can I use Nanostring microRNA count data (normalized or raw data) to measure relative gene expression by comparing it with an endogenous control (mRNA) expression?
11 November 2019 5,407 4 View
Good day! I have some predicted sequences that was not previously annotated in plant genome. Than It was found that it's possible to BLAST these sequences using RNAseq data found in ncbi SRA...
11 November 2019 5,856 3 View
It is well known that Gal4-vp16 can induce strong expression of interested genes downstream of tandom UAS moduals. I am just wondering if any one knows another equivalent system that can achieve...
11 November 2019 4,875 2 View
According to our research, we have found expression of miR-183-5p reduced in peripheral blood in patients with diabetic neuropathy, and ITGB1 is one of the targets of miR-183-5p. Therefore, its...
03 November 2019 818 3 View
I have allele freqency data for Human Leukocyte Antigen (HLA) of a specific population. Now I want to analyse Hardy Weinberg Equilibrium, Haplotype frequency and Linkage Disequilibrium for my...
20 October 2019 722 3 View
Hi all , I am confirming gene expression using RT-PCR. If a gene is down-regulated does that mean it is not expressed in the strain?
15 October 2019 5,708 3 View
10 October 2019 6,747 3 View
Good afternoon, we are currently working on a project wich seeks to investigate the NONHSAG011351 lncRNA expression in T1DM patients and healthy controls. Here we use the TaqMan expression system...
10 October 2019 9,256 3 View
Also, if I want to get genes' expression, should I have to know the gene?
10 October 2019 9,520 3 View
I'm working on a project involving gene transcription and translation rates in Klebsiella pneumoniae and Acinetobacter baumannii. Is there a codon optimization tool for non-model organisms? Most...
10 October 2019 409 2 View
I want to do ABA treatment in Arabidopsis thalliana. I am looking for some protocols in which I can dip the sample in ABA and then evaluate for some gene expression after some hours.
10 October 2019 1,550 1 View
I need to check post mycorrhizal symbiosis induced gene expression in maize roots by qRT PCR, there are lot genes like DMI, SYMRK, NUP, PHT, RAM (in Arabidopsis) but their role in maize -...
22 September 2019 2,639 2 View
in RTqPCR. if i have my target gene expression at ct 33 and beta actin at ct 17..i repeated and that's always the ratio..could my rna be degraded but i have good ct for beta actin how?
12 September 2019 3,562 3 View
09 September 2019 8,810 2 View
variants role in protien synthesis and gene expression, upto to what extent the variants are involved in regulation of gene.
09 September 2019 7,876 1 View
Hello, I am struggling with which multiple comparison correction is the best for my data. Any help would be appreciated. I have 4 variables(let's call them a,b,c and d) with 6 distributions...
29 August 2019 7,523 3 View
metformin a drug that used to reduce the insulin in peripheral blood of patients with PCOS and then reduce of endrogen ; how the metformin reduce the INS gene expression ??
17 August 2019 2,768 3 View
By evaluating the relative length of human leukocyte telomeres, we obtained results with wide variation. We did PCR-REAL-TIME. Is this normal variability? How big is the noise in this technique...
13 August 2019 9,796 0 View
08 August 2019 7,912 3 View