The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
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Questions related to Gene Expression
I am currently working with Allen brain atlas and I want to know how can I get the complete gene expression profiling of human from the atlas?
01 January 2020 2,456 2 View
I am going to inhibit Hela cell growth to see whether it can change one gene's expression? Thank you!
01 January 2020 7,667 3 View
hi, i would kindly ask you for help, because i have been strugling with this issue for few weeks. Basically i would like to know how can i check if my qRT-PCR data has normal distribution so i can...
01 January 2020 974 2 View
I'm trying to retrieve the gene expression data from different grades of brain tumor by using RERMBRANDT database. However, i could not able to do so.Anyone expertise in this tool could help me...
01 January 2020 6,488 2 View
I have two batches of samples which were collected during two time period. If I perform batch correction to remove batch effect will it affect the downstream analysis of gene expression studies?
20 December 2019 888 3 View
12 December 2019 1,406 3 View
I am studying how a marine sponge holobiont reacts to particular environmental conditions (Differential gene expression in a treatment - control study). To do so, I sequenced 12 samples that...
12 December 2019 5,136 2 View
Hi, everyone, I am using pBR- recA promoter (Inducer: formaldehyde) with GFPuv construct in E.Coli. We want to detect the GFP signal in the presence of an inducer. We used different conc. of...
12 December 2019 9,422 2 View
I performed Pearson correlation coefficient calculation (PCC) on the gene expression data and then on the principle component space (considering all components). The PCC looks different in both...
12 December 2019 7,285 3 View
Can I use Nanostring microRNA count data (normalized or raw data) to measure relative gene expression by comparing it with an endogenous control (mRNA) expression?
26 November 2019 375 2 View
Hello, I am trying to determine if a region present in a Streptomyces genome acts as a ncRNA that affects gene expression. Which are the best tools to analyze sequences for this? Thanks!
25 November 2019 5,416 3 View
Recently I ran a Microarray analysis on cells treated with purified plant-extracted compound, however, the caspase 3 levels were elevated after 24 hours suggesting high apoptotic activity of...
22 November 2019 7,248 1 View
I am using siRNA knock down to evaluate changes in gene expression in cancer cells. I found that GAPD is an unsuitable reference gene for my system given that it highly variates between...
11 November 2019 2,248 5 View
I’m new to this kind of data set, and we received data back from a company on gene expression of various genes In the form of log2 values. I’m not sure how to calculate fold change from these...
11 November 2019 5,037 5 View
I want to observe differential gene expression of cells that have a knockout of this gene versus another type of cell that normally does not express this gene. Do you know where I can find the...
11 November 2019 6,107 1 View
If I want to use high expression of my target protein (due to its low activity) in E.coli in shake flask as well as pilot scale bioreactor ? What are the factors that I must bear in mind ? Low or...
11 November 2019 1,107 1 View
11 November 2019 3,252 2 View
11 November 2019 5,371 4 View
Good day! I have some predicted sequences that was not previously annotated in plant genome. Than It was found that it's possible to BLAST these sequences using RNAseq data found in ncbi SRA...
11 November 2019 5,807 3 View
It is well known that Gal4-vp16 can induce strong expression of interested genes downstream of tandom UAS moduals. I am just wondering if any one knows another equivalent system that can achieve...
11 November 2019 4,828 2 View
According to our research, we have found expression of miR-183-5p reduced in peripheral blood in patients with diabetic neuropathy, and ITGB1 is one of the targets of miR-183-5p. Therefore, its...
03 November 2019 734 3 View
Hi all , I am confirming gene expression using RT-PCR. If a gene is down-regulated does that mean it is not expressed in the strain?
15 October 2019 5,663 3 View
10 October 2019 6,683 3 View
Good afternoon, we are currently working on a project wich seeks to investigate the NONHSAG011351 lncRNA expression in T1DM patients and healthy controls. Here we use the TaqMan expression system...
10 October 2019 9,216 3 View