The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
2,521 views 1,687 posts
Questions related to Gene Expression
Hi. I want to make a dataset for machine learning. It should take gene expression data as input, the degree of activation of pathway as output(up-regulated, down-regulated). I found a post that...
02 February 2020 4,830 8 View
I serially diluted my cDNA to generate a standard curve. For the melt curve, all the samples in the standard had one, nice peak. Except one sample in the very middle of the dilution series....
02 February 2020 8,608 3 View
Dear all, I am working on RNA-seq data from two different cancer cohorts (different ethnicity). I want to compare the expression profile of these two ethnicity. PCA showed that these two cohorts...
28 January 2020 9,177 3 View
I am currently working with Allen brain atlas and I want to know how can I get the complete gene expression profiling of human from the atlas?
22 January 2020 2,160 2 View
hi, i would kindly ask you for help, because i have been strugling with this issue for few weeks. Basically i would like to know how can i check if my qRT-PCR data has normal distribution so i can...
21 January 2020 3,544 2 View
Dear all, I am looking for the origin of the joined picture that I found on a slide server about the kinetics of gene expression (IL-2/IL-2R/CD40L) followed by proliferation after T cell...
03 January 2020 6,180 2 View
01 January 2020 7,267 2 View
Hi everyone: I am wondering can I use a multicopy gene as the reference gene for qPCR? If not, what are the potential problems involved? Thank you!
01 January 2020 3,254 4 View
Hi there, I want to use ddCt to analyze my qPCR results, however I am not exactly sure on how to proceed and when to average the replicates. Also, can we use ddCt when having more than 1 factor...
01 January 2020 1,448 6 View
Hello, I already performed my quantitative PCR (q RT-PCR). I have a lot of samples in my experiment and so they didn't fit all in the same rotor/plate (RotorGene - Qiagen). Therefore, I used a...
01 January 2020 7,828 7 View
01 January 2020 5,689 3 View
Hi. I would like to know if the absence of biological replicates can affect the qRT-PCR. I have some field samples of insects/mites in TriZOL Reagent and I plan have gene expression studies. Few...
01 January 2020 2,772 8 View
01 January 2020 2,499 2 View
I am going to inhibit Hela cell growth to see whether it can change one gene's expression? Thank you!
01 January 2020 7,721 3 View
01 January 2020 1,026 2 View
I'm trying to retrieve the gene expression data from different grades of brain tumor by using RERMBRANDT database. However, i could not able to do so.Anyone expertise in this tool could help me...
01 January 2020 6,541 2 View
I have two batches of samples which were collected during two time period. If I perform batch correction to remove batch effect will it affect the downstream analysis of gene expression studies?
20 December 2019 1,049 3 View
12 December 2019 1,500 3 View
I am studying how a marine sponge holobiont reacts to particular environmental conditions (Differential gene expression in a treatment - control study). To do so, I sequenced 12 samples that...
12 December 2019 5,185 2 View
Hi, everyone, I am using pBR- recA promoter (Inducer: formaldehyde) with GFPuv construct in E.Coli. We want to detect the GFP signal in the presence of an inducer. We used different conc. of...
12 December 2019 9,485 2 View
I performed Pearson correlation coefficient calculation (PCC) on the gene expression data and then on the principle component space (considering all components). The PCC looks different in both...
12 December 2019 7,348 3 View
Can I use Nanostring microRNA count data (normalized or raw data) to measure relative gene expression by comparing it with an endogenous control (mRNA) expression?
26 November 2019 479 2 View
Hello, I am trying to determine if a region present in a Streptomyces genome acts as a ncRNA that affects gene expression. Which are the best tools to analyze sequences for this? Thanks!
25 November 2019 5,498 3 View
Recently I ran a Microarray analysis on cells treated with purified plant-extracted compound, however, the caspase 3 levels were elevated after 24 hours suggesting high apoptotic activity of...
22 November 2019 7,277 1 View