The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
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Questions related to Gene Expression
Dear colleagues, for my research purpose i want to cross a transgenic GFP-reporter strain with a mutant strain. What is the optimal procedure to obtain a strain which inherits the transgenic...
06 July 2021 4,817 2 View
The relationship between gene expression and disease was widely known, however, is the gene ratio (Gene A expression value/Gene B expression value) might cause any diseases or phenotypes in any...
22 June 2021 5,501 5 View
Usually taqman qPCR gene expression assays using taqman fast advanced mastermix require 20ul reaction volumes. I wanted to scale down reaction volumes to 10 or 5ul to save on reagents but I was...
22 June 2021 9,318 3 View
Hi everyone, At the moment I am designing a spatial gene expression experiment using the 10X Visium assay. There are a few papers out there that have used this assay. There are also several...
16 June 2021 4,581 2 View
Gene expression in Prokaryotes and Viruses?
14 June 2021 2,977 3 View
I have transduced mammalian cells with lentivirus to express an exogenous gene. The transduced cells were sorted out on a FACS machine based on the immunostaining of the protein encoded by the...
03 June 2021 3,725 6 View
I am an undergraduate student trying to determine what features the Affymetrix HG-U133 Plus uses to measure the expression of specific genes for feature extraction. All I have so far is the level...
31 May 2021 2,156 2 View
I have identified 12 transcript variants of my gene of interest from ensembl and I want to find the expression of these transcripts in body tissues using GTEx. I think to do this however, I need...
11 May 2021 6,562 3 View
I transduced ovarian cancer cell lines (A2780 and OVCAR4 ) with pLentiCRISPRv2 having sgRNA. after puromycin selection at 0.75ug/ml concentration for 8 days I isolated RNA and converted to cDNA...
28 April 2021 7,680 4 View
If only gene expression data is available and we want to abstract mutation information of those samples/patients using their gene expression information. Is it possible? Is there any algorithm for...
09 April 2021 1,898 5 View
I'm searching for list of possible chemical compountd (Ligand) that interacts with one of the target receptor for my studies. is there any database which I could refer ? If not how to pull out the...
30 March 2021 2,198 5 View
I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...
24 February 2021 9,464 3 View
It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...
21 February 2021 6,017 3 View
My gene of interest is lowly expressed. What is the maxium amount of template (ng) one could use for RT-qPCR to detect the gene expression? I used 10 ng with no signal at 40 cycles.
10 February 2021 8,961 7 View
Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...
16 January 2021 2,649 3 View
Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...
15 January 2021 1,526 2 View
Dear Researchers, I am using pCAMBIA1301/1302 vectors for overexpression study, so can use the same vectors for transient expression? or need to design another vector for transient expression;...
09 January 2021 8,335 2 View
Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...
28 December 2020 4,109 3 View
I am looking for an online tool to see whether a certain methylated CpG site can affect the expression of a certain gene or not?
19 December 2020 7,898 1 View
Hi, I have a question if anyone can answer. I have identified two co-factors for my transcription factor. But the mass spec data seems insignificant. Now I want to find if some other co-factors...
09 December 2020 512 3 View
Hy everybody, I am looking for a way to stop gene expression during harvesting of microalgae to ensure reliable RNAseq in response to temperature. I am affraid working on ice and performing...
26 November 2020 7,485 3 View
Since our RNA is less abundant, can we use the libraries prepared for RNA-seq to validate (qPCR)the results of RNA-seq.
13 November 2020 1,046 6 View
Hi, As described, recently we found a gene was silenced in various tumor, and its promoter was alwasy hypermethlated. When using certain treatment to induce the gene expression, we found its...
05 November 2020 7,368 3 View
Hi I want to plot a graph for Ct values from qPCR assay that resulted from a gene expression in different samples.
30 October 2020 1,594 3 View