The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
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Questions related to Gene Expression
Dear community, we recently sequenced dsDNA fragments by Oxford Nanopore and currently investigate the obtained data. Since our dsDNA fragments are all in the range of 100-3000bp, we wonder if...
05 May 2020 8,743 1 View
Dear Scientists Is any database that allows me to the prediction of chromatin modifiers (like kdm6a, a histone demethylase / EZH2, a histone methyltransferase) regulate what transcriptional...
27 April 2020 526 0 View
I am trying to perform a RT-qPCR and I would like to design a primerset for one single transcript variant for RT-qPCR. The problem is that it is very difficult to get primers that only bind on one...
23 April 2020 3,312 4 View
Bats, the vectors for covid doesn’t die of covid. It is known that viral gene expression is enhanced by ultrasound (https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-8-446). As bats...
19 April 2020 8,188 3 View
I am preforming docking in AutoDock software, I choose protein and ligand, followed all the docking steps. As soon I give Run Autogrid, I will get " AutoDock needs the extension of the docking...
10 April 2020 9,937 9 View
I want to determine that how far my plants have progressed toward flowering, through the BvFT1 and BvFT2 gene expression. Should I subtract the ΔΔCt of BvFT2 from the ΔΔCt of BvFT1 or I should...
01 April 2020 5,664 1 View
When in-ovo injection of selenium, what is your suggestion about its effective role on post-hatch chicks?
25 March 2020 5,367 3 View
I've performed experiments to analyze the effect of different concentrations of a drug on gene expression using RT-qPCR. It is not clear to me whether data should be ploted as Fold Change vs....
23 March 2020 2,202 5 View
I have several study results of natural compounds which were generated by the nonostring. Since there are only limited information of these compound, I would like to set up some analysis of gene...
23 March 2020 7,043 3 View
Where can I find children leukemia dataset with patient characteristics not just gene expression?
21 March 2020 3,183 3 View
I am looking for a cheaper TagMan master mix for qPCR. I use FAM labeled primers for gene expression, does anyone have any suggestions
18 March 2020 1,332 2 View
I have applied foliar fertilizers on wheat, Now i aimed to study gene expression of wheat and want to analysis is there change occur in gene expression please guide me which method or technique...
16 March 2020 8,240 5 View
For bacterial promoters, everything I read says bacterial promoters are -35/-10 motifs upstream of the TSS, and perhaps UP elements (~ -50 of TSS). So if I want a promoter sequence for my gene,...
09 March 2020 1,107 7 View
The results showed low expression of A and overexpression of B in Tumor tissues. We expect them to show a negative correlation, but they show a significant positive correlation. Can you explain...
07 March 2020 3,339 11 View
Hi guys, I got gene expression data in normal human tissues from GTEx, Illumina, BioGPS, HPA and FANTOM5. Is there any way to combine all of these results into one? Is there such a thing? Kind...
03 March 2020 9,409 3 View
I'm getting zero or no ct value in my gene expression analysis during RT-PCR analysis. I'm not understanding the meaning of zero ct value, how I should interpret the data now. Should I consider...
03 March 2020 3,266 3 View
how is it possible to have an increase in a gene expression of a anabolic gene(agpat) and catabolic gene (hmgcs2)at the same time? may insulin affect this?
27 February 2020 6,406 1 View
how long do rats live after induction of diabetes with intraperitoneal streptozotocin injection provided the resultant hypoglycemia is taken care immediately within the succeeding 24 hours.
24 February 2020 4,618 2 View
Hello, I working with a method that finds high-expressed genes in a single-cell experiment. How can I assess such method? I know I can compare it with the ones in literature (t-test, regression),...
23 February 2020 3,234 2 View
Take a look at the notch signaling pathway in human from KEGG : https://www.genome.jp/kegg-bin/show_pathway?hsa04330 What is Fringe activating? It is not pointing to another gene or protein, it...
19 February 2020 3,362 1 View
Hi. I want to make a dataset for machine learning. It should take gene expression data as input, the degree of activation of pathway as output(up-regulated, down-regulated). I found a post that...
11 February 2020 2,855 8 View
02 February 2020 9,408 1 View
02 February 2020 8,649 2 View
To study gene expression with qPCR, I'm using 16s as a reference gene. 16s is of course very, very abundant so I need to dilute my cDNA a lot. However, I don't want to overdilute my cDNA as then...
02 February 2020 6,846 4 View