The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
2,523 views 1,687 posts
Questions related to Gene Expression
Can you perform a log transformation, i.e. Log2 on your 2^-DDCT data to reduce the skew, thereby performing parametric tests as these may be more robust and reliable than non-parametric tests.
03 March 2019 7,285 3 View
In this project that I have been given, we are to formulate a hypothesis and make a proposal as to how we would like to do experiments to test our hypothesis. One of the experiments that I will...
03 March 2019 4,725 2 View
The value of double delta ct is provided in second last corner of attached excel . And fold change value in last. please explain how we can say that the gene is upregulated or downregulated .
03 March 2019 6,804 2 View
Is it necessary to have whole genome sequence to study data CRISPR based gene expression in bacteria? what are the other requirements to initiate the process.
19 February 2019 9,734 2 View
Hello, I am using Taqman probes in RT-PCR in order to identify gene expression in genomic DNA but this expression is very low and I am trying to do it in a higher concentration of the tamplate...
11 February 2019 3,913 3 View
Hi, This seems a bit unusual to me, since I could not find any related paper. This is my situation: We have CT values (3 replicates each) for following conditions for gene A: - Wild type (WT)...
02 February 2019 5,476 6 View
02 February 2019 4,091 2 View
02 February 2019 8,557 3 View
My mastermix was over due to some proplems that happened so i need to buy new one..but I didn't find the same trade name..can I use another ? And can i compare results of both ?
02 February 2019 4,276 4 View
Hi all, I have mRNA-seq samples of two related species. What tests can I do? i have any treatment and any condition for testing. I have only mRNA-seq in control condition. Can I consider two...
02 February 2019 6,160 2 View
I have isolated total RNA from FFPE tissues but the concentration is very low (1-2 ng/uL), the amount of 2-5 ng in reaction would work with the analysis of gene expression using sybrgreen in qRT-PCR ?
29 January 2019 2,865 4 View
I cannot find good literature to support this question. I am very interested in one gene which regulate the ion channel in weakly electric fish. I currently want to make a comparison of the gene...
25 January 2019 8,032 1 View
Hello everybody, I am performing gene expression analysis using the Bio-Rad CFX maestro Software. I have different strains which I set up as "biological groups". In different plates I put my...
14 January 2019 5,957 2 View
Should it increase or decrease?
05 January 2019 8,421 1 View
01 January 2019 9,339 1 View
01 January 2019 510 1 View
Hello everyone! I am performing the analysis of gene expression level in a transformed plant and I need the derivative reporter values to perform the calculation with two normalizers. My question...
01 January 2019 614 4 View
I am looking at the difference in gene expression for a particular target gene and how this changes depending on a specific treatment. I have a house-keeping gene as a reference. I am using the...
01 January 2019 1,012 6 View
hi everyone, i am analyzing the Biological interpretation of gene expression data using cluster 3 software and visualising by heat map and clustering.i can choose the clustering method to...
01 January 2019 8,459 4 View
I want to use such a dataset for my research.
01 January 2019 6,183 4 View
Is there any difference in using doxycycline hydrochloride or doxycycline hyclate salt for doxycycline-inducible gene expression?Any special requirements for doxycycline reagents used for gene...
01 January 2019 1,498 3 View
Housekeeping genes have stable expression under different experimental conditions, but sometimes their gene expression change. I want to know why?? Particulary in Actin gene in plants.
01 January 2019 6,155 2 View
01 January 2019 6,233 1 View
If I measure gene expression in an organism under two different conditions, do I still need to normalize these genes to a housekeeping gene or comparison between the genes under two different...
19 December 2018 6,480 2 View