The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
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Questions related to Gene Expression
As we are finding difficult to secure the Trypan Blue supplies in Colombia, i would like to know if there is any alternative reagent to Stain the PBMC cells.
24 May 2023 2,965 2 View
want a brief explain for this
25 March 2023 2,085 4 View
Do you utilize Lipofectamine 3000 to transfect human splenic endothelial and fibroblast cell lines? If so, please share your transfection rate and the conditions under which you conduct the...
23 March 2023 5,597 0 View
I want to develop a model of type 2 diabetes mellitus by daily administration of sucrose in rats. but there is no verified protocol for it.
07 March 2023 5,521 0 View
How are your experiences using FSH instead of PMSG for the superovulation of mice? Is the injection scheme the same as when using PMSG? Can the diluted solution be stored below -20 as it is the...
22 February 2023 2,108 0 View
Hello, According to the attached letter It was written that "The definitions given for IFG or IGT in terms of the range of FPG were inconsistent across studies.". In table 1, I knew that The World...
16 February 2023 7,901 2 View
For MNV preparation, I inoculated MNV in Raw 267.4 cells (70 confluence) in a 6-well plate for two days, after observe CPE, I froze 6-well plate to -80C fridge for 1h and thawed at 37C water bath...
15 February 2023 6,751 2 View
Is there any website to download free DNAMAN software for mac? thanks
13 February 2023 237 4 View
Hello, I have been searching to find if when mixed together will, NA22 bacteria metabolize Amphetamine. I have had no luck finding an answer.
04 January 2023 8,918 1 View
Long story short, I am looking to do a stimulation assay in rescued knockout keratinocytes using BMP. Unfortunately for me I am running into issues with low transfection efficiency using...
03 January 2023 3,441 1 View
I have read some articles that used the double mutation to assay gene expression and function in bacteria. I do not know why we need to do this technique. Could someone help me to explain this...
17 December 2022 4,471 4 View
Hi, I would like to run southern blot, which lab provides this service. I have about 20 DNA samples with high quality and quantity, I just want to see the number of genes copies in these samples,...
09 December 2022 6,295 1 View
Hi, is there any general concensus on which would be the best 16S rRNA primers to use to cover both bacterial and archaela community? What about fungal ITS? In the literature I've came across...
01 December 2022 5,524 0 View
I was starting a PCR cleanup with QIAquick kit from QIAGEN, when I realized I was out of spin columns. I already added buffer PB (guanidine hydrochloride and isopropanol) and a little sodium...
29 November 2022 3,590 1 View
I have a question regarding the hybridisation step in Southern blotting. I understand that once the probes are added, the membrane is washed in buffer of increasing stringency to increase the...
18 November 2022 997 1 View
MicroRNAs (miRNAs) are small, single chain,non-coding RNAs (ncRNAs) of approximately 17 to 24 nucleotides in length . At the cellular level, miRNAs regulate multiple normal biological processes in...
06 November 2022 6,391 2 View
I have a series of root growth rate measurements of Arabidopsis lines carrying a single KO in gene x or gene y, and a series of measurements of lines carrying a double KO in both gene x and y. I...
04 November 2022 5,944 0 View
C2C12 cells when exposed to 2% horse serum are reported to differentiate into myotubes. I have tried 2% horse serum DMEM for 5-6 days but no myotube formation is evident. Can any one suggest the...
03 November 2022 4,293 2 View
I have a similar data and want to show it like that. Which software or R package can plot this figure. The figure is from the paper"RBM20, a gene for hereditary cardiomyopathy, regulates titin...
26 October 2022 8,542 0 View
I have seen a few CNS articles with similar recombinant protein injection strategies. The protein injection volume was 100ul, and the frequency was once every three days for a total of 8...
21 October 2022 7,346 0 View
I have used the Cdh16-cre mice hybridizing with the X gene-flox mice to generated a tubular epithelial cells-specific X gene deletion mice. There was no problem with the genotyping, the cre was...
05 September 2022 7,346 0 View
Hi, we are trying to culture cryoplateable primary hepatocytes purchased from a vendor, however, although it seemed that the viable cells were ~80% after thaw, when we seeded cells on...
17 August 2022 9,872 0 View
I am actually reading an article entitled as "Leukocyte Telomere Length Correlates With Extended Female Fertility" so I'm doubtful about length of telomere in Leukocyte and Oocyte.
19 March 2022 7,416 2 View
Hi all, I recently conducted a kinase activity array experiment, using the PamGene Chip. Now we have the data showing us which kinases are more or less active in our treated vs control. We want...
16 December 2021 6,468 1 View