The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
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Questions related to Gene Expression
Is there any website to download free DNAMAN software for mac? thanks
13 February 2023 158 4 View
Long story short, I am looking to do a stimulation assay in rescued knockout keratinocytes using BMP. Unfortunately for me I am running into issues with low transfection efficiency using...
03 January 2023 3,383 1 View
I have read some articles that used the double mutation to assay gene expression and function in bacteria. I do not know why we need to do this technique. Could someone help me to explain this...
17 December 2022 4,410 4 View
Hi, I would like to run southern blot, which lab provides this service. I have about 20 DNA samples with high quality and quantity, I just want to see the number of genes copies in these samples,...
09 December 2022 6,233 1 View
Hi, is there any general concensus on which would be the best 16S rRNA primers to use to cover both bacterial and archaela community? What about fungal ITS? In the literature I've came across...
01 December 2022 5,464 0 View
I have a question regarding the hybridisation step in Southern blotting. I understand that once the probes are added, the membrane is washed in buffer of increasing stringency to increase the...
18 November 2022 934 1 View
MicroRNAs (miRNAs) are small, single chain,non-coding RNAs (ncRNAs) of approximately 17 to 24 nucleotides in length . At the cellular level, miRNAs regulate multiple normal biological processes in...
06 November 2022 6,331 2 View
I have a series of root growth rate measurements of Arabidopsis lines carrying a single KO in gene x or gene y, and a series of measurements of lines carrying a double KO in both gene x and y. I...
04 November 2022 5,846 0 View
C2C12 cells when exposed to 2% horse serum are reported to differentiate into myotubes. I have tried 2% horse serum DMEM for 5-6 days but no myotube formation is evident. Can any one suggest the...
03 November 2022 4,224 2 View
I have a similar data and want to show it like that. Which software or R package can plot this figure. The figure is from the paper"RBM20, a gene for hereditary cardiomyopathy, regulates titin...
26 October 2022 8,492 0 View
I have seen a few CNS articles with similar recombinant protein injection strategies. The protein injection volume was 100ul, and the frequency was once every three days for a total of 8...
21 October 2022 7,284 0 View
I have used the Cdh16-cre mice hybridizing with the X gene-flox mice to generated a tubular epithelial cells-specific X gene deletion mice. There was no problem with the genotyping, the cre was...
05 September 2022 7,284 0 View
Hi, we are trying to culture cryoplateable primary hepatocytes purchased from a vendor, however, although it seemed that the viable cells were ~80% after thaw, when we seeded cells on...
17 August 2022 9,811 0 View
I am actually reading an article entitled as "Leukocyte Telomere Length Correlates With Extended Female Fertility" so I'm doubtful about length of telomere in Leukocyte and Oocyte.
19 March 2022 7,349 2 View
Dear colleagues, for my research purpose i want to cross a transgenic GFP-reporter strain with a mutant strain. What is the optimal procedure to obtain a strain which inherits the transgenic...
06 July 2021 4,767 2 View
The relationship between gene expression and disease was widely known, however, is the gene ratio (Gene A expression value/Gene B expression value) might cause any diseases or phenotypes in any...
22 June 2021 5,452 5 View
Usually taqman qPCR gene expression assays using taqman fast advanced mastermix require 20ul reaction volumes. I wanted to scale down reaction volumes to 10 or 5ul to save on reagents but I was...
22 June 2021 9,247 3 View
Hi everyone, At the moment I am designing a spatial gene expression experiment using the 10X Visium assay. There are a few papers out there that have used this assay. There are also several...
16 June 2021 4,536 2 View
Gene expression in Prokaryotes and Viruses?
14 June 2021 2,903 3 View
I have transduced mammalian cells with lentivirus to express an exogenous gene. The transduced cells were sorted out on a FACS machine based on the immunostaining of the protein encoded by the...
03 June 2021 3,667 6 View
I am an undergraduate student trying to determine what features the Affymetrix HG-U133 Plus uses to measure the expression of specific genes for feature extraction. All I have so far is the level...
31 May 2021 2,113 2 View
I have identified 12 transcript variants of my gene of interest from ensembl and I want to find the expression of these transcripts in body tissues using GTEx. I think to do this however, I need...
11 May 2021 6,524 3 View
I transduced ovarian cancer cell lines (A2780 and OVCAR4 ) with pLentiCRISPRv2 having sgRNA. after puromycin selection at 0.75ug/ml concentration for 8 days I isolated RNA and converted to cDNA...
28 April 2021 7,623 4 View
If only gene expression data is available and we want to abstract mutation information of those samples/patients using their gene expression information. Is it possible? Is there any algorithm for...
09 April 2021 1,848 5 View