The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
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Questions related to Gene Expression
I have transduced mammalian cells with lentivirus to express an exogenous gene. The transduced cells were sorted out on a FACS machine based on the immunostaining of the protein encoded by the...
03 June 2021 3,658 6 View
I am an undergraduate student trying to determine what features the Affymetrix HG-U133 Plus uses to measure the expression of specific genes for feature extraction. All I have so far is the level...
31 May 2021 2,102 2 View
I have identified 12 transcript variants of my gene of interest from ensembl and I want to find the expression of these transcripts in body tissues using GTEx. I think to do this however, I need...
11 May 2021 6,515 3 View
I transduced ovarian cancer cell lines (A2780 and OVCAR4 ) with pLentiCRISPRv2 having sgRNA. after puromycin selection at 0.75ug/ml concentration for 8 days I isolated RNA and converted to cDNA...
28 April 2021 7,610 4 View
If only gene expression data is available and we want to abstract mutation information of those samples/patients using their gene expression information. Is it possible? Is there any algorithm for...
09 April 2021 1,837 5 View
I'm searching for list of possible chemical compountd (Ligand) that interacts with one of the target receptor for my studies. is there any database which I could refer ? If not how to pull out the...
30 March 2021 2,124 5 View
I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...
24 February 2021 9,226 3 View
It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...
21 February 2021 5,814 3 View
My gene of interest is lowly expressed. What is the maxium amount of template (ng) one could use for RT-qPCR to detect the gene expression? I used 10 ng with no signal at 40 cycles.
10 February 2021 8,755 7 View
Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...
16 January 2021 2,455 3 View
Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...
15 January 2021 1,311 2 View
Dear Researchers, I am using pCAMBIA1301/1302 vectors for overexpression study, so can use the same vectors for transient expression? or need to design another vector for transient expression;...
09 January 2021 8,123 2 View
Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...
28 December 2020 3,897 3 View
I am looking for an online tool to see whether a certain methylated CpG site can affect the expression of a certain gene or not?
19 December 2020 7,703 1 View
Hi, I have a question if anyone can answer. I have identified two co-factors for my transcription factor. But the mass spec data seems insignificant. Now I want to find if some other co-factors...
09 December 2020 440 3 View
Hy everybody, I am looking for a way to stop gene expression during harvesting of microalgae to ensure reliable RNAseq in response to temperature. I am affraid working on ice and performing...
26 November 2020 7,360 3 View
Since our RNA is less abundant, can we use the libraries prepared for RNA-seq to validate (qPCR)the results of RNA-seq.
13 November 2020 919 6 View
Hi, As described, recently we found a gene was silenced in various tumor, and its promoter was alwasy hypermethlated. When using certain treatment to induce the gene expression, we found its...
05 November 2020 7,173 3 View
Hi I want to plot a graph for Ct values from qPCR assay that resulted from a gene expression in different samples.
30 October 2020 1,492 3 View
Hi everyone, I struggle to find a single-cell or bulk-sequencing dataset showing the transcriptomic effects of a medical treatment (i.e. COX1 or 2 -inhibition) in patients of inflammatory...
29 October 2020 5,607 3 View
Currently, I am validating a new machine learning approach. But, I need to find different cancer datasets composed by clinical markers and gene expressions. For now, a just found MMRF data set...
12 October 2020 9,126 2 View
Good day, I hope you are all doing well. I was designing primers for Caspase 9 and sometimes, the designed primers in NCBI have transcript variants for the same gene (Caspase 9) but at the same...
06 October 2020 550 3 View
how to calculate the value for adjusted p value in gene expression analysis? what is the formula? i'm working on identifiying the level of expression of lncRNA from a datasets deposited in NCBI....
27 September 2020 9,668 4 View
I am having trouble navigating the phylofish database. I can blast my gene of interest and get the " Best match," but from there, I cant seem to figure out where to go to find the expression level...
16 September 2020 5,005 2 View