By evaluating the relative length of human leukocyte telomeres, we obtained results with wide variation. We did PCR-REAL-TIME. Is this normal variability? How big is the noise in this technique for measuring telomere length?
I want to know more about Uranium ore deposits in world.
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I want to know more about diamond ore deposits in world.
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We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
10 August 2024 6,678 14 View
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,198 5 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
What are a “Farmers Producer Organization” (FPO) and its essential features?
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I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
08 August 2024 4,831 4 View
How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
08 August 2024 8,162 0 View
I am currently culturing leukocytes from TLRE filters from our blood bank. I have been struggling to culture these cells, as well as improve their viability. I was wondering if anyone would be...
16 June 2024 3,752 0 View
I am trying to measure telomere length in human DNA samples through qPCR. In the protocol, they have not included extension step. Can anybody explain how a qPCR can work without an extension step?...
31 May 2024 514 2 View
Dear Friends! The gold-standard for identifying an allele for human leukocyte antigen is SBT or sequence-based typing. If I devise a new PCR test or LAMP test, how many known positive and negative...
16 February 2024 7,953 1 View
Hello, I am trying to visualize a genomic locus. I produced Alexa fluor 488 dUTP tagged FISH probes by nick translation. I used two PCR amplified DNA fragments (12kb and 16 kb) that span through...
26 January 2024 1,080 1 View
I am a bit confused about this question. I am currently attempting to do a meta-analysis on pesticide exposure and telomere length (outcome). The exposure(s) were basically presented as either a...
17 January 2024 3,267 3 View
Hi all, I am interested in knowing if there are antibodies that I can use to detect fibrosis in blood cells using flow cytometry. Can anyone with expertise in immunology and flow cytometry please...
29 November 2023 405 1 View
I am prepping whole blood for flow cytometry analysis of leukocytes and using BD Red Blood Cell lysis buffer (15m mins then spinning off), but my RBCs don't seem to lyse. The solution remains a...
29 October 2023 8,097 3 View
I’m currently conducting research on telomeres in THP-1 cells. During my gel electrophoresis experiments using a 1% gel, I’ve encountered an issue where the DNA seems to be stuck at the top of the...
29 October 2023 9,561 7 View
Hello, First, let me describe the problem I'm trying to solve. I transformed yeast with an antibiotic marker to truncate a chromosome, with the fragment having a homologous sequence on one and...
05 August 2023 3,416 2 View
What should be the length of telomere after treating a Particular MCF-7 cell line with a plant extract? It should decrease or increase the length of telomere? is there any particular value for...
10 March 2023 9,172 1 View