The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
2,522 views 1,687 posts
Questions related to Gene Expression
Hi everyone, I struggle to find a single-cell or bulk-sequencing dataset showing the transcriptomic effects of a medical treatment (i.e. COX1 or 2 -inhibition) in patients of inflammatory...
29 October 2020 5,698 3 View
Currently, I am validating a new machine learning approach. But, I need to find different cancer datasets composed by clinical markers and gene expressions. For now, a just found MMRF data set...
12 October 2020 9,306 2 View
Good day, I hope you are all doing well. I was designing primers for Caspase 9 and sometimes, the designed primers in NCBI have transcript variants for the same gene (Caspase 9) but at the same...
06 October 2020 695 3 View
I was just wondering if anyone knows of a way to compare whether there is a significant increase or decrease in a fitted GAM model between factors. I currently have nominal data that is separated...
01 October 2020 3,105 1 View
how to calculate the value for adjusted p value in gene expression analysis? what is the formula? i'm working on identifiying the level of expression of lncRNA from a datasets deposited in NCBI....
27 September 2020 9,767 4 View
I am having trouble navigating the phylofish database. I can blast my gene of interest and get the " Best match," but from there, I cant seem to figure out where to go to find the expression level...
16 September 2020 5,104 2 View
whats the difference between RNA extraction(with trisol)when we want to measure long non coding RNA and when we want to measure gene expression?
08 September 2020 7,607 5 View
Hi, im newbee for analysing gen expression data set and hope someone have som advise. Im working on mice liver gen expression (mRNA) data sets generated with EdgeR - now I want to do some gen...
04 September 2020 1,456 3 View
I adopt primer designs from a study published in Nature ( Domestication selected for deceleration of the circadian clo... ), so the primers should be qualified. Yet in many samples, I got...
01 September 2020 10,012 8 View
I am trying to analyze the micro array gene expression data between control and treatment. I would like to use R programming package. Which package is better and how can i use. I want step by step...
24 August 2020 8,425 7 View
Hello everyone. I am an undergraduate student majoring in cardio-thoracic technology. I'm now working on my Bachelor's thesis that i use real time PCR (gene expression study between drug-treated...
12 August 2020 4,822 9 View
Does anyone know how this number became "default"? what is it based on? How deep is our knowledge of "average" , "healthy" gene expression of different cell types? Sorry if my question is trivial...
05 August 2020 7,389 1 View
I have five different groups for single cell sequence analysis. I only want a particular gene expression in fouror three groups, Can anyone please help me how to code?
02 August 2020 319 2 View
i want to study the expression of NF-kB and TGF-b and STAT3 in flow cytometry sorted MDSCs cells (case vs control) and in tumor tissue in order to determine their activation status in...
31 July 2020 7,681 2 View
I have pated 1 million cells on a coverslip in a 6 well dish to perform immunocytochemistry. Now I have to plate the same in a normal 6 well dish to study gene expression. Please suggest to me how...
31 July 2020 7,650 8 View
I have knocked down a gene of interest using siRNA and am using qRT-PCR to confirm reduced expression. All samples treated with non-specific siRNA have Ct values around 35, but all samples...
30 July 2020 8,536 3 View
I am following Cawthon 2009 method, with 2 targets amplified in one well. Single copy gene (albumin) amplification in the same well is great. As the machine used for this experiment is Roche...
28 July 2020 4,937 0 View
Hi, In LogNormalize function feature counts for each cell are divided by the total counts for that cell and multiplied by the scale.factor prior to log transformation. My question is what does...
27 July 2020 2,253 2 View
I have more than 500 Datasets of RNA-sequencing data (both FASTA and FASTQ format) and I'd like to analyze gene expression and differentially expressed genes. files with FASTA format are in my PC...
15 July 2020 3,606 3 View
Hi everyone, I'm trying to establish a standard protocol to cultivate BMDMs. I'm culturing BM cells for 7 days in 100mm petri dishes, addition of M-CSF(20ng/mL) on day 0 and day 3. I would like...
10 July 2020 2,037 3 View
Hi , this is Qunting . I am working on the direction of single-cell library construction base on Tn5 transposase. Now I have a problem with my work , I sincerely ask for your help. My single cell...
09 July 2020 5,532 0 View
At sea-level, stress-induced (non-exercise, psycho-physiological) hypo/hyper -oxemia causes an alteration in resting VO2, RQ, VE, end-tidal gases, and partial pressure of blood-gases as well. How...
05 July 2020 2,411 0 View
Hello all, I am working on genes affected in cholesterol metabolism in patients with Coronary Artery Disease and PCSK9 is one such target gene in my study. I have managed to obtain stable results...
14 June 2020 7,250 9 View
resently we submited some samples from zebrafish to do a rnaseq (Novogene), the problem was that due to our low understanding of the process, we got a denovo analysis (for species without a...
07 May 2020 3,651 3 View