The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
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Questions related to Gene Expression
Does anyone know how this number became "default"? what is it based on? How deep is our knowledge of "average" , "healthy" gene expression of different cell types? Sorry if my question is trivial...
05 August 2020 7,073 1 View
I have five different groups for single cell sequence analysis. I only want a particular gene expression in fouror three groups, Can anyone please help me how to code?
02 August 2020 254 2 View
i want to study the expression of NF-kB and TGF-b and STAT3 in flow cytometry sorted MDSCs cells (case vs control) and in tumor tissue in order to determine their activation status in...
31 July 2020 7,623 2 View
I have pated 1 million cells on a coverslip in a 6 well dish to perform immunocytochemistry. Now I have to plate the same in a normal 6 well dish to study gene expression. Please suggest to me how...
31 July 2020 7,595 8 View
I have knocked down a gene of interest using siRNA and am using qRT-PCR to confirm reduced expression. All samples treated with non-specific siRNA have Ct values around 35, but all samples...
30 July 2020 8,437 3 View
I am following Cawthon 2009 method, with 2 targets amplified in one well. Single copy gene (albumin) amplification in the same well is great. As the machine used for this experiment is Roche...
28 July 2020 4,897 0 View
Hi, In LogNormalize function feature counts for each cell are divided by the total counts for that cell and multiplied by the scale.factor prior to log transformation. My question is what does...
27 July 2020 2,154 2 View
I have more than 500 Datasets of RNA-sequencing data (both FASTA and FASTQ format) and I'd like to analyze gene expression and differentially expressed genes. files with FASTA format are in my PC...
15 July 2020 3,538 3 View
Hi everyone, I'm trying to establish a standard protocol to cultivate BMDMs. I'm culturing BM cells for 7 days in 100mm petri dishes, addition of M-CSF(20ng/mL) on day 0 and day 3. I would like...
10 July 2020 1,908 3 View
Hi , this is Qunting . I am working on the direction of single-cell library construction base on Tn5 transposase. Now I have a problem with my work , I sincerely ask for your help. My single cell...
09 July 2020 5,495 0 View
Hello all, I am working on genes affected in cholesterol metabolism in patients with Coronary Artery Disease and PCSK9 is one such target gene in my study. I have managed to obtain stable results...
14 June 2020 7,191 9 View
resently we submited some samples from zebrafish to do a rnaseq (Novogene), the problem was that due to our low understanding of the process, we got a denovo analysis (for species without a...
07 May 2020 3,614 3 View
Dear community, we recently sequenced dsDNA fragments by Oxford Nanopore and currently investigate the obtained data. Since our dsDNA fragments are all in the range of 100-3000bp, we wonder if...
05 May 2020 8,704 1 View
Dear Scientists Is any database that allows me to the prediction of chromatin modifiers (like kdm6a, a histone demethylase / EZH2, a histone methyltransferase) regulate what transcriptional...
27 April 2020 474 0 View
I am trying to perform a RT-qPCR and I would like to design a primerset for one single transcript variant for RT-qPCR. The problem is that it is very difficult to get primers that only bind on one...
23 April 2020 3,204 4 View
Bats, the vectors for covid doesn’t die of covid. It is known that viral gene expression is enhanced by ultrasound (https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-8-446). As bats...
19 April 2020 8,095 3 View
I am preforming docking in AutoDock software, I choose protein and ligand, followed all the docking steps. As soon I give Run Autogrid, I will get " AutoDock needs the extension of the docking...
10 April 2020 9,870 9 View
I want to determine that how far my plants have progressed toward flowering, through the BvFT1 and BvFT2 gene expression. Should I subtract the ΔΔCt of BvFT2 from the ΔΔCt of BvFT1 or I should...
01 April 2020 5,581 1 View
When in-ovo injection of selenium, what is your suggestion about its effective role on post-hatch chicks?
25 March 2020 5,306 3 View
I've performed experiments to analyze the effect of different concentrations of a drug on gene expression using RT-qPCR. It is not clear to me whether data should be ploted as Fold Change vs....
23 March 2020 2,079 5 View
I have several study results of natural compounds which were generated by the nonostring. Since there are only limited information of these compound, I would like to set up some analysis of gene...
23 March 2020 6,969 3 View
Where can I find children leukemia dataset with patient characteristics not just gene expression?
21 March 2020 3,142 3 View
I am looking for a cheaper TagMan master mix for qPCR. I use FAM labeled primers for gene expression, does anyone have any suggestions
18 March 2020 1,223 2 View
I have applied foliar fertilizers on wheat, Now i aimed to study gene expression of wheat and want to analysis is there change occur in gene expression please guide me which method or technique...
16 March 2020 8,178 5 View