The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
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Questions related to Gene Expression
Hello! I'm following the protocol for isolation of human granulocytes from peripheral blood found in chapter 3 of Neutrophil: Methods and Protocols. They recommend using 3% Dextran to sediment red...
last month 9,655 1 View
… men come to the sexual experience with only his porno-sex-ed and general socialization – ergo pleasing a woman means: 1) A big penis 2) Lasting a long time during intercourse 3) Making her...
26 April 2025 7,389 2 View
If you’ve got a penis, it would be super convenient if the way the person with a vagina has pleasure is for you to put your penis in their vagina. (Emily Nagoski)
13 April 2025 4,251 0 View
•Why the above mentioned ratios are maintained? is this ratio resemble the breast tumor microenvironment? can anyone suggest the coculture ratio of Jurkat/T cell : MCF-7 for any T cell mediated...
17 January 2025 4,770 0 View
Gene editing could extend human lifespan by: Enhancing DNA repair to slow aging. Reducing cellular senescence to remove aging cells. Boosting longevity genes like FOXO3. Modulating telomeres to...
19 August 2024 6,755 2 View
cq values for my reference coming like 22 and cq value of my target gene coming as 23 and 24 around? can i considered this value for future calculation? or what else i can do to get better cq...
15 May 2024 6,113 6 View
04 March 2024 7,165 0 View
I have synthesized recombinant plasmid with my gene of interest. I need to insert the recombinant plasmid into algal cells. Please suggest experimentation literature for the same.
01 December 2023 9,433 4 View
Why choose PNA-HPLC methods for divalent siRNA detection over PCR?
30 November 2023 1,224 1 View
Does the founder effect increase genetic diversity and why do cheetahs have very little genetic variation?
29 October 2023 7,535 1 View
We are trying to perform mouse liver biliary tree（bile duct) staining, is there a detailed protocol to follow? Any help is highly appreciated. Warm Regards, Liang
09 October 2023 7,961 2 View
Hi everyone. I'm Dr. Abu Sayeed from Bangladesh. I'm an Epidemiologist and public health researcher from North South university Mph Program. I've devised an assignment on the topic of "Vietnam's...
29 September 2023 9,436 3 View
Does natural selection cause adaptation and can mutation increase genetic variation and relationship among evolutionary mechanisms and genetic diversity?
18 September 2023 8,195 5 View
i need a pcr condiditon for ERK, JNK and MMP1 genes. i have tried many but still i didnt get the correct band. Can anyone have information about this?
13 September 2023 3,673 0 View
To save people’s lives, a warning (forecast) must be given hours before the start of the tremors. Gordon Woo, do you see this? You recommend the EEWS method and have banned the publication of a...
09 September 2023 5,211 2 View
How to plant timely in inadequate soil moisture situations in zero till system and why planking is to be avoided in zero till planted wheat?
04 August 2023 2,333 1 View
Genetic engineering has the potential to be used for curing genetic diseases in humans. The field of genetic engineering encompasses various techniques that involve modifying an organism's genetic...
20 June 2023 256 1 View
I have used IDT Oligoanalyzer for checking my primers. In here, the accepted delta G value for internal self‐dimers(-9kcal/mol) and the 3' end (-6kcal/mol) has been mentioned but not for the 5'end...
19 June 2023 3,649 1 View
I´m creating a phylogenetic tree algorithm that will classify species according it's beta globine of hemoglobin and sequences, but I' don't know how to deal with sequences of different lengths.
19 June 2023 7,923 2 View
After running pcr reaction (for 35 cycle) the dna concentration i got was low. Is it possible to use same tube to run it again (for 35 cycle or any)? what would happen if i do that?
17 June 2023 3,387 6 View
How does PCR conditions affect specific target ?
15 June 2023 3,264 3 View
From what I understand, Fst looks for heterozygosity at a specific loci, and therefor can't be used to analyze mtDNA sequences. However, I see Fst being used all the time in papers dealing with...
07 June 2023 3,065 1 View
How is spironolactone dissolved for oral gavaging in mice?
31 May 2023 260 0 View
Hi, I try to clone a V5-TurboID (1Kb) into a roughly 6kb big backbone (pEGFPC2). The restriction enzymes used are NheI and XhoI. Both fragments are gel purified and ligated with T4 Ligase (1h or...
31 May 2023 6,285 4 View