The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
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Questions related to Gene Expression
cq values for my reference coming like 22 and cq value of my target gene coming as 23 and 24 around? can i considered this value for future calculation? or what else i can do to get better cq...
15 May 2024 6,098 6 View
I have synthesized recombinant plasmid with my gene of interest. I need to insert the recombinant plasmid into algal cells. Please suggest experimentation literature for the same.
01 December 2023 9,404 4 View
Why choose PNA-HPLC methods for divalent siRNA detection over PCR?
30 November 2023 1,205 1 View
Does the founder effect increase genetic diversity and why do cheetahs have very little genetic variation?
29 October 2023 7,522 1 View
We are trying to perform mouse liver biliary tree（bile duct) staining, is there a detailed protocol to follow? Any help is highly appreciated. Warm Regards, Liang
09 October 2023 7,938 2 View
Hi everyone. I'm Dr. Abu Sayeed from Bangladesh. I'm an Epidemiologist and public health researcher from North South university Mph Program. I've devised an assignment on the topic of "Vietnam's...
29 September 2023 9,414 3 View
Does natural selection cause adaptation and can mutation increase genetic variation and relationship among evolutionary mechanisms and genetic diversity?
18 September 2023 8,180 5 View
i need a pcr condiditon for ERK, JNK and MMP1 genes. i have tried many but still i didnt get the correct band. Can anyone have information about this?
13 September 2023 3,648 0 View
To save people’s lives, a warning (forecast) must be given hours before the start of the tremors. Gordon Woo, do you see this? You recommend the EEWS method and have banned the publication of a...
09 September 2023 5,193 2 View
How to plant timely in inadequate soil moisture situations in zero till system and why planking is to be avoided in zero till planted wheat?
04 August 2023 2,314 1 View
Genetic engineering has the potential to be used for curing genetic diseases in humans. The field of genetic engineering encompasses various techniques that involve modifying an organism's genetic...
20 June 2023 241 1 View
I have used IDT Oligoanalyzer for checking my primers. In here, the accepted delta G value for internal self‐dimers(-9kcal/mol) and the 3' end (-6kcal/mol) has been mentioned but not for the 5'end...
19 June 2023 3,625 1 View
I´m creating a phylogenetic tree algorithm that will classify species according it's beta globine of hemoglobin and sequences, but I' don't know how to deal with sequences of different lengths.
19 June 2023 7,899 2 View
After running pcr reaction (for 35 cycle) the dna concentration i got was low. Is it possible to use same tube to run it again (for 35 cycle or any)? what would happen if i do that?
17 June 2023 3,365 6 View
How does PCR conditions affect specific target ?
15 June 2023 3,229 3 View
From what I understand, Fst looks for heterozygosity at a specific loci, and therefor can't be used to analyze mtDNA sequences. However, I see Fst being used all the time in papers dealing with...
07 June 2023 3,034 1 View
How is spironolactone dissolved for oral gavaging in mice?
31 May 2023 248 0 View
Hi, I try to clone a V5-TurboID (1Kb) into a roughly 6kb big backbone (pEGFPC2). The restriction enzymes used are NheI and XhoI. Both fragments are gel purified and ligated with T4 Ligase (1h or...
31 May 2023 6,267 4 View
As we are finding difficult to secure the Trypan Blue supplies in Colombia, i would like to know if there is any alternative reagent to Stain the PBMC cells.
24 May 2023 2,947 2 View
want a brief explain for this
25 March 2023 2,069 4 View
Do you utilize Lipofectamine 3000 to transfect human splenic endothelial and fibroblast cell lines? If so, please share your transfection rate and the conditions under which you conduct the...
23 March 2023 5,583 0 View
I want to develop a model of type 2 diabetes mellitus by daily administration of sucrose in rats. but there is no verified protocol for it.
07 March 2023 5,510 0 View
Hello, According to the attached letter It was written that "The definitions given for IFG or IGT in terms of the range of FPG were inconsistent across studies.". In table 1, I knew that The World...
16 February 2023 7,889 2 View
For MNV preparation, I inoculated MNV in Raw 267.4 cells (70 confluence) in a 6-well plate for two days, after observe CPE, I froze 6-well plate to -80C fridge for 1h and thawed at 37C water bath...
15 February 2023 6,741 2 View