The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
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Questions related to Gene Expression
Genetic engineering has the potential to be used for curing genetic diseases in humans. The field of genetic engineering encompasses various techniques that involve modifying an organism's genetic...
20 June 2023 167 1 View
I have used IDT Oligoanalyzer for checking my primers. In here, the accepted delta G value for internal self‐dimers(-9kcal/mol) and the 3' end (-6kcal/mol) has been mentioned but not for the 5'end...
19 June 2023 3,541 1 View
After running pcr reaction (for 35 cycle) the dna concentration i got was low. Is it possible to use same tube to run it again (for 35 cycle or any)? what would happen if i do that?
17 June 2023 3,281 6 View
How does PCR conditions affect specific target ?
15 June 2023 3,117 3 View
From what I understand, Fst looks for heterozygosity at a specific loci, and therefor can't be used to analyze mtDNA sequences. However, I see Fst being used all the time in papers dealing with...
07 June 2023 2,945 1 View
How is spironolactone dissolved for oral gavaging in mice?
31 May 2023 179 0 View
Hi, I try to clone a V5-TurboID (1Kb) into a roughly 6kb big backbone (pEGFPC2). The restriction enzymes used are NheI and XhoI. Both fragments are gel purified and ligated with T4 Ligase (1h or...
31 May 2023 6,187 4 View
want a brief explain for this
25 March 2023 1,990 4 View
For MNV preparation, I inoculated MNV in Raw 267.4 cells (70 confluence) in a 6-well plate for two days, after observe CPE, I froze 6-well plate to -80C fridge for 1h and thawed at 37C water bath...
15 February 2023 6,663 2 View
Is there any website to download free DNAMAN software for mac? thanks
13 February 2023 143 4 View
Long story short, I am looking to do a stimulation assay in rescued knockout keratinocytes using BMP. Unfortunately for me I am running into issues with low transfection efficiency using...
03 January 2023 3,372 1 View
I have read some articles that used the double mutation to assay gene expression and function in bacteria. I do not know why we need to do this technique. Could someone help me to explain this...
17 December 2022 4,399 4 View
Hi, I would like to run southern blot, which lab provides this service. I have about 20 DNA samples with high quality and quantity, I just want to see the number of genes copies in these samples,...
09 December 2022 6,220 1 View
Hi, is there any general concensus on which would be the best 16S rRNA primers to use to cover both bacterial and archaela community? What about fungal ITS? In the literature I've came across...
01 December 2022 5,451 0 View
MicroRNAs (miRNAs) are small, single chain,non-coding RNAs (ncRNAs) of approximately 17 to 24 nucleotides in length . At the cellular level, miRNAs regulate multiple normal biological processes in...
06 November 2022 6,315 2 View
C2C12 cells when exposed to 2% horse serum are reported to differentiate into myotubes. I have tried 2% horse serum DMEM for 5-6 days but no myotube formation is evident. Can any one suggest the...
03 November 2022 4,213 2 View
I have a similar data and want to show it like that. Which software or R package can plot this figure. The figure is from the paper"RBM20, a gene for hereditary cardiomyopathy, regulates titin...
26 October 2022 8,480 0 View
I have seen a few CNS articles with similar recombinant protein injection strategies. The protein injection volume was 100ul, and the frequency was once every three days for a total of 8...
21 October 2022 7,273 0 View
I have used the Cdh16-cre mice hybridizing with the X gene-flox mice to generated a tubular epithelial cells-specific X gene deletion mice. There was no problem with the genotyping, the cre was...
05 September 2022 7,272 0 View
Hi, we are trying to culture cryoplateable primary hepatocytes purchased from a vendor, however, although it seemed that the viable cells were ~80% after thaw, when we seeded cells on...
17 August 2022 9,799 0 View
The relationship between gene expression and disease was widely known, however, is the gene ratio (Gene A expression value/Gene B expression value) might cause any diseases or phenotypes in any...
22 June 2021 5,441 5 View
Usually taqman qPCR gene expression assays using taqman fast advanced mastermix require 20ul reaction volumes. I wanted to scale down reaction volumes to 10 or 5ul to save on reagents but I was...
22 June 2021 9,236 3 View
Hi everyone, At the moment I am designing a spatial gene expression experiment using the 10X Visium assay. There are a few papers out there that have used this assay. There are also several...
16 June 2021 4,525 2 View
Gene expression in Prokaryotes and Viruses?
14 June 2021 2,892 3 View