The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. | Contact experts in Gene Expression to get answers
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Questions related to Gene Expression
I am looking for user friendly bioinformatic software that help me out in predicting SNPs effect on a gene expression ?
08 August 2019 7,516 3 View
Simply put, I would like a measure of variability in gene expression within the gtex dataset. I would like a measure that can be compared between genes so that I can rank them from most variable...
08 August 2019 6,327 4 View
We have an RNA-Seq dataset with two measurements (concentration and weight) and would like to know how these two factor influence our gene expression. To solve this question we would like to...
08 August 2019 6,423 5 View
Is foliar application of amino acids and fruits peel extracts on wheat can make any change in their gene expression of wheat grains
26 July 2019 443 3 View
I have three C. elegans lines with the same transcriptional RFP reporter inserted in the same site via MosSCI. Two of them show an expression pattern that match what I would expect based WormBase,...
07 July 2019 4,776 2 View
I am currently designing a host-vector system for heterologous gene expression in a filamentous fungus.
07 July 2019 7,078 3 View
Sometimes some gene gets over expressed in organism, so which molecular technique can help us to detect the over expression of that gene?
07 July 2019 9,518 5 View
I have several microarray gene expression datasets, but they use different naming systems for the probes. What are some simple ways to convert all the probes to the same type (e.g. Affymetrix)?...
07 July 2019 10,029 3 View
is 1.2 fold change a good cut off for differential gene expression using microarray?
07 July 2019 8,640 5 View
Working using RT-qPCR, we need to use house keeping genes with constant gene expression as endogenous references to quantify expression of target genes. I am wondering how the house keeping genes...
07 July 2019 3,876 7 View
07 July 2019 7,886 4 View
I'm trying to understand epigenetic variability but the study I'm performing involves measuring the gene expression in a given region according to the SNP's alleles associated with the increase of...
23 June 2019 6,658 12 View
i have been doing qPCR analysis for some Pathogenicity related genes in Fungus but when i start calculating the fold changes using given protocol. the expression of control become 1 always. can...
14 June 2019 8,231 8 View
I have a CreLoxP mouse that expresses cre recombinase, upon treatment with tamoxifen with subsequent deletion of floxed genes. I will hippocampal neurons from conditional knockout mouse and aim to...
12 June 2019 878 0 View
06 June 2019 1,466 9 View
06 June 2019 6,717 11 View
I performed a real time pcr and would like to compare the time-dependent gene expression of the control group among the results. e.g. between Control 0h/ Control 24h/ Control 48h Can I use the...
06 June 2019 4,593 8 View
I am currently preparing to do RNAseq for the first time and am having trouble obtaining a copy of 'RNA‐Seq for Bacterial Gene Expression' (2018) by Line Dahl Poulsen & Jeppe Vinther from...
06 June 2019 3,495 5 View
I'm doing a RT-PCR on orchids, but the program I use now takes up to 3 hours. It would be nice if I could reduce that time with Phire. However, I'm not sure if it affects the gene-expression. Are...
06 June 2019 5,758 5 View
dear all as it is my first time to design a taqman assay , could you recommend for me a good reference to know the good designing for taqman gene expression assay, what is the accepted range for...
16 May 2019 8,393 2 View
I'm trying to compare caloric intake of patients with colon cancer with the calculated ideal caloric intake. What statistical test should I use? assuming data are normally distributed should I use...
12 May 2019 6,506 2 View
05 May 2019 3,103 2 View
I mean promoter-reporte analysis of regulation of gene expression
05 May 2019 9,700 3 View
I want to compare gene expression between 3 patients and 3 controls to investigate if there is any difference between the two groups. I've ran a RT-qPCR using SYBR Green. Should I use RQ values?...
05 May 2019 7,367 4 View