whats the difference between RNA extraction(with trisol)when we want to measure long non coding RNA and when we want to measure gene expression?
Ideally, there should be no difference in the extraction between different types of RNA. The RNA precipitation can be modified by using different alcohols (isoproyl, ethyl), but these changes should also yield only marginal differences in extraction of RNA.
The differences are mainly introduced in later steps, after the extraction of total RNA. For instance, poly-A-based selection (to get rid of the large amounts of ribosomal RNA in any mammalian sample) is suitable for mRNA analysis, but removes large amounts of non-coding RNA. So, if you want to measure lncRNA, ribosomal depletion would be more suitable.
Hello
You dont need to change anything during RNA extraction. Although, you would consider some modifications if you were after small RNAs.
Good luck
Emanuel A Devers Hi sir you mean I should wash the pellet with 80%ethanol for extraction of long non coding RNA?thanks for your answer
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