• From your spread sheet you cannot
• There is astandard method called the Livak method for calculating relative gene expression changes
• I have made refernce to exactly how you do this in a prior answer of mine:
• https://www.researchgate.net/post/How_can_I_calculate_gene_expression_levels_with_RT-PCR#view=54994b14cf57d76a568b45a3
• In essence, you normalise expression for a treated and untreated sample by taking away the housekeeping Ct for that sample (Delta Ct)
• You then subtract the untreated sample normalised (delta Ct value) from the treated delta Ct value (giving you a so called Delta Delta Ct value)
• To calculate fold change in expression of your gene in a treated sample relative to the untreated control or calibrator you then use the delta delta Ct value as an exponent of 2; that is 2^ (Delta Ct Treated - Delta Ct control)
• I have attached the original Livak paper and worked examples for you to adapt to your own situation

Thanks Laurence Stuart Dawkins-Hall