There are various software/tools available that help in designing primers for genome editing. It is important to know the target site and based on that, primers can be designed to alter the gene of interest. However, sometimes there will be off target mutation, it needs to keep in mind.

Could you tell me how do i check the compatibility (of plasmids available in addgene) with my bacteria. For example the overexpression plasmids/vectors used in other bacteria (also for someother gene), will it work for me? or how do i select one?