How does SEA software normally number subsystems?

Dear colleagues, What is the common subsystem numbering system in SEA modelling software (i.e. VA One, AutoSEA2)? Cheers, Feng

27 February 2021 7,073 3 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Where can I find these two papers?

14 January 2021 1,572 3 View

After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

Asalam o alikum, Can anyone please help me in interpretation of XRD data?

Actually, I want to present the in table form (dcal , dexp, 2 thita, hkl) but don't know how to find these values from data.

28 December 2020 6,769 4 View

Is it need to test Cronbach's Alpha for extracted features from data?

As Cronbach's Alpha is usually used for test the reliability of measurements given by questionnaires, however, I wonder that whether I use this test for features extracted from behavioral data. I...

26 December 2020 9,602 2 View

Doping of r-GO?

if the percentage of doping Au into rGO is too small 1~10%, is it not enough to prevent restacking of r-GO in xrd results, rGO peak appeared at 22.2 and also the peaks of gold nanoparticles so...

21 December 2020 6,091 3 View

How to extract dry soils such as rocks and Debris from Sentinel-2 images using Google Earth Engine?

How to extract dry soils such as rocks and Debris from Sentinel-2 images using Google Earth Engine?

17 December 2020 6,600 2 View

XRD peaks for gold nanoparticles ?

is the concentration of gold salt that were added will affect on the peaks appeared. i added a very little amount of gold salt to dope graphene oxide and not all the peaks that proved the...

17 December 2020 9,275 3 View

If someone has the pdf file of below mentioned reseach paper?

14 December 2020 5,935 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View