Hi,

I would like to express a toxic construct in HeLa and 293T cells. It has to be easily inducible and absolutely not leaky. I did some research and only few promoters are available:

Tet promoter seems to be leaky. Several papers claimed to have improved the system such as this one PMID: 27323850 that claim to have optimized the tet promoter with leakiness below detectable levels or this one PMID: 33378396. Did anyone test one of these?

Is the Cumate-based system leaky?

Something else?

Thank you.

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