How to calculate the qPCR amplicon size for a given assay target from a given primer set along with a hydrolysis probe?

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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Ahmed Elsadek Fakhr

If you know the primer sequence of your primers you can know the length of products through blast analysis.

Hydrolysis probe should not affect the product size as the probe is hydrolyzed not the amplicon.

Sven Reister

probe binds between the primers, wouldn't work in another way with Taqman assays. so the primers drive the amplicon size