Protocol for Coomassie Brilliant Blue (G-250) for Total Protein Normalisation?

22 January 2023 0 10K Report

Hello! My skeletal muscle biology lab investigates the effect Notch signaling has on muscle repair. We have had issues with the housekeeping protein signal (Beta-actin) being too irregular. I have been reading about total protein normalization, and it seems like it would be a good idea for our studies.

When is the best time to stain the membrane? Before or after immunodetection?

Please let me know if you have a protocol you would not mind sharing with me!

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