I have read many protocols for the preparation of cross-linked hyaluronic acid gel for soft tissue fillers, but cross-linking active ingredients are hard to find in my country, so I am looking for alternatives.
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
From a population of transfected/transduced cells I sort those with the desired expression. Starting from 10.000 cells I extract the ARN, and from its cDNA I amplify the gene of interest, as well...
19 September 2020 8,378 3 View
I believe by using greater number of antennas in simulation, SNR would also be improved. However I have no proof for that. Are there any equations that support my idea?
25 September 2019 4,890 3 View
the DSC results for a blend containing 90% HDPE and 10% polyethylene wax shows two separate peaks during the first heating, one for the wax phase and one for the HDPE phase. but the peak...
08 May 2019 3,580 7 View
I want to design a horn antenna which only produces incident field along theta (it's got copolarization along theta). how can I do that?
05 February 2019 9,911 3 View
Hi! I use this protocol: Purification and Properties of an Extraeellular Endo-l,4-Glucanase from Lenzites trabea (Herr, et al 1978), but i would like to know if there are protocols standardized by...
13 August 2018 252 2 View
The viral RNA obtained from a patient with influenza symptoms was analyzed and the results for a real-time PCR were negative for the influenza A virus and positive for the AH1NI virus. The...
05 July 2018 9,009 3 View
I want to avoid any contamination between tissues
08 March 2017 4,160 2 View
Using a matlab package to calculate effect sizes (MEStoolbox), I chose to evaluate g_psi for the effect sizes between a control group and 12 treatments. In a previous boxplot of samples, I can...
06 November 2012 9,336 4 View
In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...
02 March 2021 8,149 3 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...
22 February 2021 9,896 3 View
I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...
18 February 2021 6,736 4 View