I have read many protocols for the preparation of cross-linked hyaluronic acid gel for soft tissue fillers, but cross-linking active ingredients are hard to find in my country, so I am looking for alternatives.
What are the recommended fluorophores for in vivo tumor imaging using the IVIS system? I am particularly interested in options compatible with IVIS imaging and would like information on the limit...
19 November 2023 4,004 0 View
There are a lot of factors that can contribute in bacterial antibiotic resistance. What do you think is the most significant factor in bacterial antibiotic resistance?
23 February 2023 7,770 7 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 5,466 3 View
From a population of transfected/transduced cells I sort those with the desired expression. Starting from 10.000 cells I extract the ARN, and from its cDNA I amplify the gene of interest, as well...
19 September 2020 8,523 3 View
I believe by using greater number of antennas in simulation, SNR would also be improved. However I have no proof for that. Are there any equations that support my idea?
25 September 2019 5,070 3 View
the DSC results for a blend containing 90% HDPE and 10% polyethylene wax shows two separate peaks during the first heating, one for the wax phase and one for the HDPE phase. but the peak...
08 May 2019 3,832 7 View
I want to design a horn antenna which only produces incident field along theta (it's got copolarization along theta). how can I do that?
05 February 2019 10,013 3 View
Hi! I use this protocol: Purification and Properties of an Extraeellular Endo-l,4-Glucanase from Lenzites trabea (Herr, et al 1978), but i would like to know if there are protocols standardized by...
13 August 2018 350 2 View
I am currently in the process of deciding on a research question (and an according method/research design) for my master thesis in the named field. So far, I have not been able to find an open...
06 August 2018 5,928 3 View
Hello everyone, I'm looking for the factors which have an impact on the quality of blood or blood cells, like WBCs/PBMCs. For example, I know that after blood draw, granulocytes may cause...
06 August 2018 1,268 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,478 3 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,685 1 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,129 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
My collegue and I are having hard time to understand what is happening. Basically we have a tapping device that work with Arduino and a pneumatic pump. The pump allows the tapping of two...
04 August 2024 4,661 1 View
Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...
31 July 2024 3,911 6 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,404 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 949 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,662 6 View