How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to obtain free lipids starting from concentration over the CMC?

I need to work with free lipids at increasing concentration (50%, 60% of the CMC ect.). I'm now working with POPC and POPG. To reach these concentrations I have to start from concentration over...

17 February 2021 1,676 3 View

Why CMC Agar medium must incubated on 2x24 hours or 3x24 hours?

As we known that CMC Agar medium is used for cellulolitic bacteria or fungi which have different time on incubation

06 January 2021 3,515 2 View

How can I dissolve Sodium carboxy methyl cellulose powder in Normal Saline solution and water?

I need this solution for my research work. I am facing problem in dissolution of CMC powder

30 December 2020 5,157 2 View

How to make SiO2 layer long lasting?

I make three layer solar cell (TiO2-SiO2-TiO2/SiO2) with doctor blade method but SiO2 layer broke all layer when it dry. I have add some adhessive like PVA, CMC, or sodium silicate but the result...

12 October 2020 630 6 View

What if the abbreviations of two technical terms in the manuscript are the same?

I'm wrting an article, there are two terms "Carboxymethylcellulose" and "Critical Micelle Concentratio" both have the abbreviations of "CMC". How should i distinguish them?

23 June 2020 2,115 5 View

Visual representation of water, acetonitrile (solvent), SDS (surfactant)?

Hello, Just wondering if anyone knows what this mixture would look like when stable and when mixed? I.e. how do the molecules distribute. Can assume that SDS is below the CMC and water and...

27 April 2020 5,578 3 View

Does anyone know a compound which can be used to increase the viscosity of an aqueous solution such that the viscosity is stable over a range of pHs?

I plan to used a Bourne reaction system or similar to study reactive mixing. I wish to vary the viscosity of the system between experiments to understand how the viscosity influences the reactive...

07 April 2020 9,058 4 View

Why is the Phase inversion temperature method of emulsification not working?

Hello, I am currently conducting an experiment that involves oil in water emulsions. I have read various literature regarding the creation of microemulsions by bringing the solution of water oil...

09 March 2020 6,453 4 View

Reproducible aqueous polymer solutions?

Would greatly appreciate input from the experts. Wanted - aqueous polymer solutions with reproducible viscosity behaviour. Observation - viscosity of carboxymethyl cellulose sodium salt - CMC...

02 March 2020 1,784 1 View

Please how do i seperate the oil residue from the crude biosurfactant so i extract the biosurfactant from my bacteria-free supernatant?

I incubated my bacteria for biosurfactant production with olive oil. after centrifuging the bacteria out, I noticed some oil residue on the surface of the supernatant, trapped with the crude...

24 November 2019 6,822 2 View