Hi,
I work in research right now but I used to work in the medical field, and when I created standard dilutions for viral assay controls I would make a concoctions using I believe 10mM Tris-HCL, cRNA, and Tween 20. I do not have access to my calculations for that anymore and I want to make a similar buffer using 1X TE and Tween 20 for my qPCR control standards. We've been buying them but they are expensive and it seem unnecessary if we could just create our own from a high positive sample. Would anyone have any advice for making this if I want the total buffer volume to be 50mL?
You can make a 10X TE buffer. From there, u can make 1X TE.
Required components for 1L 10X TE Buffer:
- Tris-Cl (desired pH) (MW: 157.594 g/mol) 15.759 g
- EDTA (pH 8) (MW: 292.24 g/mol) 2.92 g
From this stock, you can make 1X
M1V1=M2V2 M1 = stock concentration [10x] V1 = volume needed of M1 concentrated stock (Z) M2 = desired concentration [1x] V2 = desired volume [say 10 ml you want to make] So as per M1V1=M2V2
10x * Zml = 1x *50ml
Z = 5 ml of 10x you need in 50 ml of water.
This also means you need to add 50-5=45 ml of water in 5 ml of 10x concentrate on making 1x buffer.
IMPORTANT Don't add 5 ml in to 50 ml. which would be 55 ml, and your concentration will be wrong.
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