Can anyone explain me how to make 5% DMSO solution?

I want to make 5% DMSO solution for my substrate such as Fatty acids and naphthalene to dissolve.

04 April 2019 2,311 6 View

Protocol for extraction and derivitization of fatty acid for GC analyis?

I want to extract my P450 reaction mixture and derivitized for GC analysis. I have read about BSTFA method, but i have no idea about the procedure. Please help me to understand the procedure.

26 January 2019 7,689 11 View

Himalayan Black Salt?

Its origin, antioxidant property?

23 January 2019 1,526 3 View

How to fuse two independent domain?

I have two independent domain cloned in E.coli and i want to fuse them both just to get a single domain by PCR. How to get this by PCR?

10 October 2018 1,718 4 View

Facing problem in Cloning?

facing problem in cloning.No colony in transformants plate.

19 August 2018 6,226 9 View

How to extract the cytoplasmic content from human cells ?

Dear all, I want only cytoplasmic content from the human cells to test my compounds. Does anyone knows how to do it or can share the protocol for doing it ?

29 June 2018 9,042 2 View

How I can measure the contraction and expansion of graphene nano particles reinforced in Copper matrix composite?

I have sintered Copper matrix composite which is reinforced by graphene, since graphene shows negative thermal expansion upto a certain temperature and copper shows positive thermal expansion...

04 February 2018 7,835 1 View

What is the effect of CuAl2O4, CuMo2S3 and AlB12 compounds on the Tribology of Copper matrix composite?

During XRD i found the peaks of CuAl2O4, CuMo2S3, AlB12 compounds and i want to know the effect of these compounds on tribological properties of Copper matrix composite during dry sliding. Plz...

16 November 2017 307 2 View

How we can measure the Tensile Strength of a Pellet?

We have a pellet of size 30 mm diameter and 6 mm. thickness,it is a metal matrix composite, how i am supposed to measure the tensile strength of it, by which machine or facility or method it can...

18 July 2017 7,362 4 View

How to identify BL21 vs BL21(DE3) strain?

How to identify BL21 vs BL21(DE3) strain?

22 May 2017 4,209 4 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How would I use a glycerol fixation to fix whole brain Drosophila tissue?

I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.

21 February 2021 3,081 3 View

How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Problem to dissolve 50%wt Glycerol in 4%wt PVOH 28-99 ??

Hello everyone, I am trying to prepare a solution out of PVA(Polyvinyl alcohol)_Glycerol_Wasser . (4% PVA 28-99 and 50% Glycerin) To prepare this solution I first add PVA to 80degree water and...

06 February 2021 2,300 3 View

What is the easiest way to remove the water from organic solvents?

Hello everyone! I have one question. I have couple of solvents and all of them were pure when they were bought but after some months or years of storage after opening I am not 100% sure about...

03 February 2021 2,154 8 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

How to simulate enzymatic hydrolyis of sucrose in a CSTR coupled with ultrafiltration membrane with comsol?

Dear all, I am trying to simulate the enzymatic hydrolysis of sucrose with invertase in CSTR in Comsol. The reaction pathway is such that Sucrose + Water -> Fructose + Glucose. It is desired...

27 January 2021 2,525 4 View