PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

The litter effect and what can I do to avoid it?

My thesis involves experimental study with Wistar puppies. I am dividing the puppies equally in relation to the groups. However I only have 4 mothers and I will use all of their puppies. There is...

25 January 2021 6,965 3 View

Since I am design automatized grader for forest seeds, it is important for me to know the following?

1. Is there a difference between a dormant seed, for example, of Scots pine, a fossilized dead seed, and an active live one by spectrometric or other characteristics? 2. Is it possible to...

26 November 2020 9,275 2 View

Should I use anesthetics before guillotine If I inteed to see brain inflammation?

Hello, I also want to do some biochemical analyses and need to take some blood of the rats. To take the blood I need to use anesthetics. But I want to evaluate TNF-a and oxidative stress in the...

19 October 2020 3,835 3 View

How does annealing affect the crystallinity and hardness of a blended wax product?

Dear all, I'm hoping to receive some feedback and tips so that I can better understand the behaviour of a material I am working with: The material is a blend of paraffin and microcrystalline...

14 August 2020 1,605 3 View

How to extract data Night Light?

24 March 2020 8,301 2 View

What is the best way to interpret and analyze a phylogenetic tree built from categorical functional traits ?

I have built a phylogenetic tree using categorical functional traits, such as pollination and dispersion syndromes, phenology, etc. Now, I can see the dicotomies and the points in which the plant...

24 February 2020 6,051 3 View

Can someone help with a creep in steel under fire conditions modelling in Ansys APDL?

Hello everyone. I am encountering some problems while chasing after the convergence of my model. I am using Ansys APDL in order to model the creep in steel under high temperatures. I already did...

06 January 2020 575 3 View

Does phase 2 clinical trials need a superiority margin?

23 July 2019 5,158 3 View

No title

I am developing a research, using as a method the modeling of structural equations, which aims to identify the determinants of sustainable consumption, or conscious consumption. However, I am...

24 November 2018 7,287 10 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

Where can I find Plasmopara viticola?

Hello everyone, For my research I want to infect Vitis Vinifera leaves with downy mildew-causing fungus Plasmopara viticola. Does anyone know where I could find or purchase this fungi? I am...

04 February 2021 5,720 3 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

Cutoff for asv reads?

I got my fungi sequences back from Illumina Miseq sequencing and I'm wondering how many reads do you use as a cutoff? ie. do you delete anything with less than 100 reads? I can't seem to find any...

28 January 2021 5,646 4 View