Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

Can I use breaking series as a regressor in a VAR model?

Context: I have two series, price and sales. Sales is mean-reverting stationery but price is stationary only after controlling for an intercept break. I want to set up a 2-equation VAR model...

14 January 2021 6,824 12 View

Does the use of Tissue Level implants reduce the risk of Peri-implantitis, as opposed to using bone level?

12 December 2020 6,007 6 View

Will the plasmid without SV40 Ori have a quite low expression level in 293T?

SV40 Ori helps plasmid to replicate in 293T cells. If I use a plasmid which doesn't have a SV40 Ori, will the expression level be very low?

10 December 2020 1,751 2 View

How to calculate the normalized frequency V of photonic crystal fiber using COMSOL?In particular, how to calculate NEFF and nFSM using COMSOL?

24 November 2020 967 2 View

HOW to do an intention-to-treat analysis(ITTA)?

I mean, how do I put the dropped out participants' outcome (Missing value) in my analysis? Although I have already known what's ITTA meaning, I still don't know how to do it. Which methods should...

23 November 2020 9,982 5 View

Does pEGFP plasmid have G418 resistant?

Instruction from pEGFP: The recombinant EGFP vector can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected usingG418 ....

20 October 2020 4,662 2 View

The solubility of ethinyl estradiol (EE2) in methanol?

What is the solubility of ethinyl estradiol (EE2) in methanol? In the experiment, methanol was used to help dissolve, what is the reason for the flocs appearing after the storage solution is added...

20 September 2020 4,055 1 View

How to compare methylation status between different cell lines?

As in the title. I got the methylation status of several genes in different cell lines by bisulfite sequencing. More specifically, I have the sequencing data from the amplicons of these genes...

30 August 2020 6,020 2 View

How can I digest the formaldehyde (1%) crosslinked DNA-protein with restriction enzyme (DpnII) better?

I am working on yeast samples for 4C-seq study , but the formaldehyde fixed samples can not be digested with restriction enzyme (DpnII). Please help me out. Thanks.

28 August 2020 6,483 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How to call both UMAT and UEL together in Abaqus?

I have a model with bulk material (matrix) and inclusions and cohesive interface elements between both. For the bulk material, I have implemented a UMAT and for the cohesive interface elements, I...

20 February 2021 1,286 2 View

How can we study the adsorption efficiency of microgel at the oil-water interface?

I am studing the mircogel at oil-water interface. I want to know the adsorb amount of microgel at the oil water interface. How we can determine this amount?. I will highly appreciate your...

19 February 2021 2,787 1 View

Can someone please tell me how to get a desired temperature (say 700C at the bar concrete interface ) which is at a depth of 15mm??

Can someone please tell me how to get a desired temperature (say 700C at the bar concrete interface ) which is at a depth of 15mm from the exposed surface in Abaqus... i am performing a coupled...

10 February 2021 4,656 2 View

In Abaqus, is it possible to remove elements which are too complex for meshing from a sequence obtained by getByBoundingBox() ?

Hello everyone, I'm trying to mesh a structure with complex partitions using mesh controls. When doing it on the user interface I'm first selecting all cells. In the following, I have to accept...

03 February 2021 8,198 1 View

How to calculate the polysaccharide content of a curcuma species through HPLC method?

what all standards are needed to quantify and analyze the composition with a proper method fie for the mobile phase to run the sample ?

31 January 2021 7,704 4 View

How to extract polysaccharids from Aloe gel?

I 'm trying to extract polysacharides from Aloe leaf and tried ethanol percipitation, but after getting the pellet. I used the protocol from following reference following the steps, but I don't...

27 January 2021 7,049 5 View

I am working with crack propagation along with delamination using ABAQUS. Wether crack will start from a delaminated portion (cohesive crack) by XFEM?

I am modelling a SENB Beam with an interface. The interface is modelled with cohesive elements to permit delamination. The carck in the continumm is taken care by the XFEM. My doubt is the crack...

19 January 2021 4,827 1 View

Can we use the single-elimination tournament method as the basis of an experiment design?

I want to conduct an experiment in the field of Human-Computer Interaction, where I test the perception of users about which image is better using different camera lenses. In the experiment, I...

18 January 2021 779 2 View

Why are nanobubbles typically negatively charged?

Nanobubbles of air in water tend to have a negative charge. Intuitively, one could think the water is not charged, air is not charged: why are nanobubbles charged? Looking closer, water has both...

14 January 2021 3,563 3 View