Cell infection with lentivirus: GFP+ after soring by flow but no protein expression?

I use a plasmid to overexpress 1 protein in 293T and murine BMSC. But I could never succeed. I produced the lentivirus by transfecting the plasmids in HEK293FT cells, together with another 3for...

24 December 2020 6,389 3 View

I keep getting an error for GEOPIV on Matlab?

When doing the geoPIV_RG_run, i keep encountering this error. Error using dataread Trouble reading floating point number from file (row 1, field 2) ==> Dynamic Load Test Free Vibration Error...

20 October 2020 10,004 2 View

Is lentiviral production of antibodies in 293T viable?

Hi, I recently started using lentiviral systems to produce proteins in mammalian cells. Knowing the performance of this impressive system in producing high titers of proteins, I am wondering if...

05 August 2020 5,144 2 View

What solutions do you suggest for molecular field visualization/analysis?

Hello, I wonder what options exist these days for visualizing "molecular field" type data. That is, a 3D scalar field represented by translucent volume rendering or isosurfaces, and overlaid on a...

12 June 2020 861 2 View

How to choose collective variables for ligand binding to protein?

Hello! What is the recommended way to choose collective variables / reaction coordinates to study ligand binding to a protein (e.g., for PMF free energy profiling)? I often see the advice like "Do...

10 March 2020 1,878 4 View

No title

02 November 2019 8,943 3 View

Why an SD of means difference between two groups (e.g. treatment vs. placebo) is equal to a particular SD in each group?

Very frequently in papers, devoted to parallel clinical trials, I face a situation, when a calculated SD for an effect in each group is approximately equal to an SD of effect difference between...

07 July 2019 9,217 4 View

What aproach we must use to calculate force in electrostatic problems: potential or electric field intensity?

Well known problem of finding potential for metal sphere and point charge, solving with "mirror image" trick give you 2 different answers for the force, acting on point charge. One when we use...

14 December 2016 9,365 5 View

Does choline hydroxide (46% aqueous) ionic liquid still absorb water from surroundings?

I will be using this ionic liquid as an electrolyte for my battery experiments.

01 August 2016 2,559 1 View

Hall Sensor and EMC?

What would be your brief recommendations for EMC for Hall sensors? I mean; how to increase robustness of the Hall sensors circuits? Any links? Thank you, Eugene

27 April 2016 8,388 9 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Can I ask did any good platform or journals for learning the calcluation on the reference governors and command governor?

Recently I face some problems with the mathematical ( calculation) on the reference governor and command governor, so I need some help from you all to get which platform or journal can let me...

23 February 2021 7,746 2 View

Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

What is the better software for approximation of 3D surface with equations?

I have a massive of x, y, z coordinates of 2D surface in 3D space, which builded in Ansys HFSS or AutoCAD. How can I get the analitical aproximation of this surface by formulas?

21 February 2021 3,444 7 View

How can I obtain a sine function along a path with known equation?

I have a symmetric airfoil with known equation. Now a sine function passes along the airfoil equation like following figure. I want to find the sine function in (x,y) coordinate system.

17 February 2021 1,594 12 View

Does learning mathematics exclusively improve cognitive abilities?

Teaching Mathematics at school to all students is commonly justified by the opinion that it improves their problem-solving skills and "makes them smarter" (whichever measure is implied by this). I...

15 February 2021 7,391 7 View

What will happen when we assume an upper bound of OFV lower than the global minimum OFV ?

if we assume 20 is the upper bound of an objective function (OF) based on permutation a square matrix (nxn) , The minimum value of the OF=22, then we run a random operator to compute the OF by...

14 February 2021 6,056 5 View