I am using KinTek software to do a kinetic fitting. I have determined kinetics of the formations of several species in the time course of 5min and established a model for the enzyme (just as an example: A = B = C = D). I already know that B is always below the detection limit of the instrument during the reaction. Is there a way to restrict the amount of B to lower than the detection limit (such as 0.01 uM) in the software? I only find restrictions for kinetic constants and the starting concentration for species. If there is not a way, is it appropriate to import a sudo-data set (such as a constant amount of 0.005 uM B at each time point, with a sigma value of 0.005) for fitting?
If B is undetectable, then maybe you should leave it out of the model entirely. See whether you can fit the data just as well with A = C = D. You don't want to have more parameters in the model than can actually be distinguished kinetically.
Adam B Shapiro Dr. Shapiro, thank you for your response. Maybe I am using an inappropriate example, but B is actually a branched point in my real model, so I can't get rid of it
I use for fitting and analysis of kinetic data the Copasi software (free download from Copasi.org ). It's very powerful and friendly for users. It allows to put restrictions on almost all parameters. You may try. If you try, please, let me know which software is better for you.
I think you can specify a concentration of each species in the model in the Kintek program, but I don't know of any way of specifying a minimum or maximum value.
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