Do you know an enzymatic unmasking method in immunohistochemistry for matrix protein?

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

How to remove the bubbles in Collagen gel and maintain the transparency?

I am working on the capillary formation of Lymphatic endothelial cells in a collagen matrix. But I am facing a technical problem to handle Collagen. I have made many times; I am not able to remove...

27 December 2020 5,495 3 View

How to calculate enzyme's energy of activation without rate constant (+excel)?

I want to calculate an enzyme's energy of activation, but I don't have the enzyme's molecular weight to calculate the rate constant or the pre-exponential factor A. This is part of an assignement...

25 December 2020 8,738 3 View

How to determine the dominant orientation of collagen fibers.?

what is the best method to determine the dominant orientation of collagen fibers? i preformed FFT and shited the frequencies , what should be my next step ?

24 December 2020 3,360 3 View

Is anyone share your experience in making hydro gel using bovine collagen?

dear all, Actually, I m trying to prepare hydrogel using fish collagen, rat tail collagen, and bovine collagen to check the cell adhesion and viability. fish and rat tail collagen nicely formed as...

15 December 2020 7,142 4 View

How to stain prostate tissue embedded in epoxy resin with Masson's trichrome?

Hi, My study involves tissue grinding of prostate tissue embedded in epoxy resin. Then performing Masson's trichrome staining for collagen on the exposed face of the grinded block face, but...

07 December 2020 6,624 2 View

What is the best Machine learning method to extract collagen bundles features ?

what is the best machine learning method , to segment and extract the length , angle and the size of collagen bundles pictures obtained from the human cornea?

07 December 2020 336 6 View

Do I need collagenase to dissociate hepatocytes?

Hi all, after culturing hepatocytes in a monolayer on collagen in 24 well plates for 5 days, I attempted to trypsinize (0.25% trypsin) them to perform a cell count. However, none of my cells...

11 November 2020 2,478 2 View

Frozen spheroid section is not staining (fluorescent + H&E), any ideas?

I have cryosectioned a spheroid which was grown in collagen and agarose and then embedded in OCT Tissue Tek solution before freezing in a dry ice ETOH bath of liquid N2. The sections are very...

08 November 2020 3,299 3 View

Why does the concentration of collagen (hydroxyproline) not consistent each extraction?

We have been extracting hydroxyproline from marine animals and the extraction was repeated several times. However, the results were not consistent and sometimes it turned out 0. We had tried the...

28 October 2020 8,164 1 View