Do you know an enzymatic unmasking method in immunohistochemistry for matrix protein?

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How to improve results of system identification procedure?

Hello, I'm performing system identification of the lateral closed-loop dynamics of a quadrotor. My model receives a setpoint and should return position and acceleration. I've proposed a second...

07 February 2021 9,791 2 View

What is the maximum time duration allowed between photoresist coating and exposure?

I have coated the Silicon wafer with photoresist (AZ1518) but until the exposure (Laser Writer) I had to wait for more than a week (Laser Writer was not operative) - would the photoresist still be...

03 January 2021 9,640 4 View

What would happen if all nitrogen atoms in DNA were replaced by Carbon atoms ?

The DNA molecule is in the form of a double helix, The atoms which compose it are carbon, nitrogen, hydrogen, and phosphorus

02 January 2021 4,402 3 View

Visualization of NaCl molecule on TEM?

I have a powder composed of NaCl molecule and I want to know the technical approach to visualise it on Transmission Electron Microscopy so I can see the shape and the form, is it possible?

02 December 2020 7,292 5 View

Which is more important for a journal? The impact factor or the quartile ranking?

I always found confusing both concepts! Which one do you think is more important? I give an example: The journal of "Theoretical and Applied Climatology" has an impact factor of 2.88, but it's a...

01 December 2020 8,608 19 View

Who can collaborate with us?

Salam (Hello)! We have prepared some new compounds with important potential to inhibit viruses. We are interested in inhibition of Hepatetis-B/C, Nipah, Nil and coronavirus. Who can collaborate...

22 November 2020 3,850 14 View

What is the most effective way to transfect HAP1 cells?

I am starting a project with the HAP1 cell line and previously our lab has had difficulty to transfect them. -we have nucleofection equipment - but have only been getting ~10% transfection -i...

19 October 2020 5,340 2 View

What effect do epidemics have on cerebral physiology?

How trauma and fear of infection can affect brain functions and structures?

10 October 2020 6,642 1 View

What are the XRD analysis conditions of the cement, sand and Limestone ?

I am a Phd student and I need to do XRD analysis on my pouder samples namely ( cement- sand- limestone and calcined clay). I was asked asked to specify the analysis conditions: -the nature of...

07 October 2020 5,737 6 View

Why I couldn't obtain a gel from APTES? Does the Amine group affect the gelation process?

I am trying to make a gel from APTES as a precursor using the sol-gel reaction. I tried different solvents (ethanol, acetone, DMF) and different catalysts (basic and acidic) with different...

03 October 2020 9,673 5 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

How to remove the bubbles in Collagen gel and maintain the transparency?

I am working on the capillary formation of Lymphatic endothelial cells in a collagen matrix. But I am facing a technical problem to handle Collagen. I have made many times; I am not able to remove...

27 December 2020 5,495 3 View

How to calculate enzyme's energy of activation without rate constant (+excel)?

I want to calculate an enzyme's energy of activation, but I don't have the enzyme's molecular weight to calculate the rate constant or the pre-exponential factor A. This is part of an assignement...

25 December 2020 8,738 3 View

How to determine the dominant orientation of collagen fibers.?

what is the best method to determine the dominant orientation of collagen fibers? i preformed FFT and shited the frequencies , what should be my next step ?

24 December 2020 3,360 3 View

Is anyone share your experience in making hydro gel using bovine collagen?

dear all, Actually, I m trying to prepare hydrogel using fish collagen, rat tail collagen, and bovine collagen to check the cell adhesion and viability. fish and rat tail collagen nicely formed as...

15 December 2020 7,142 4 View

How to stain prostate tissue embedded in epoxy resin with Masson's trichrome?

Hi, My study involves tissue grinding of prostate tissue embedded in epoxy resin. Then performing Masson's trichrome staining for collagen on the exposed face of the grinded block face, but...

07 December 2020 6,624 2 View

What is the best Machine learning method to extract collagen bundles features ?

what is the best machine learning method , to segment and extract the length , angle and the size of collagen bundles pictures obtained from the human cornea?

07 December 2020 336 6 View

Do I need collagenase to dissociate hepatocytes?

Hi all, after culturing hepatocytes in a monolayer on collagen in 24 well plates for 5 days, I attempted to trypsinize (0.25% trypsin) them to perform a cell count. However, none of my cells...

11 November 2020 2,478 2 View

Frozen spheroid section is not staining (fluorescent + H&E), any ideas?

I have cryosectioned a spheroid which was grown in collagen and agarose and then embedded in OCT Tissue Tek solution before freezing in a dry ice ETOH bath of liquid N2. The sections are very...

08 November 2020 3,299 3 View

Why does the concentration of collagen (hydroxyproline) not consistent each extraction?

We have been extracting hydroxyproline from marine animals and the extraction was repeated several times. However, the results were not consistent and sometimes it turned out 0. We had tried the...

28 October 2020 8,164 1 View