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Questions related to Dyes
Could we use ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for the protein concentration, again after washing with buffer or with mili Q water?
28 April 2022 9,255 0 View
Hi, I am looking for an appropriate dye to stain my live cells that are being cultured in scaffolds containing FITC. Does anyone have experience with dyes that excite and emit at different...
04 April 2022 1,966 0 View
I was wondering if anyone could relate their experience with isolating and analysing protein complexes in complex samples (whole-cell lysates) using mass spectrometry on sucrose gradient...
17 March 2022 9,967 5 View
Hi everyone! I'm performing an ex vivo experiment with tumor tissue slices. My idea is to inyect nanoparticles into the slice and, in orther to know where exactly did the inyection take place,...
08 February 2022 3,797 0 View
Hello RG experts I am working on dye degradation and looking for dyes which are suitable for todays scenario. Could you all please enlist some of the most contaminant dyes that are suitable for...
29 January 2022 1,313 7 View
Hi Research Community, I am looking for a method to label dendritic spines in fixed hippocampal brain slices. Due to our fixation Golgi staining seems to be off the table. Now I found Dil...
19 January 2022 5,985 2 View
I'm not interested in kinetics, but I have problems with unspecific binding of antibody labelling in very sparse in vitro cortical neuron cultures. Can the CellAura fluorescent antagonists be used...
12 January 2022 995 0 View
I'm looking for a fluorescent dye for the single molecule experiment with DNA. The fluorescence dye will be labeled into a DNA. Since it is not for the FRET experiment, only the intensity and...
07 January 2022 8,244 0 View
Greeting for everybody My question for estimating cooling load for any building consist of lighting so how can calculate the cooling load for lighting ( LED lighting) with my respect
25 December 2021 7,472 3 View
Dear All I am growing MCF10 A cells in the ATCC recommended media (DMEM: F12, 5%Horse serum, etc). I was just wondering if anyone has used the DMEM (or RPMI) with 10% FBS for the same cells? If...
21 December 2021 664 0 View
Because I want to make B16F10 cells proliferate faster in vitro, I wonder if 20%FBS will be better. Thank you.
02 December 2021 6,464 3 View
I've been using very low concentrations of Methylene blue in my MFC experiments and noted overtime binding of it on my Nafion membrane. Has anyone else experienced this?
01 December 2021 5,181 1 View
I want to determine the velocities of particles passing through a microchannel. I tried using PIVLab for this, but it gives wrong velocity profiles. That's why I used manualtrack imagej for...
28 November 2021 4,507 4 View
I have analyze two similar structure in SAP2000. Eigen value of one structure is more then other. What it actually means of when eigen value is increased ?
26 November 2021 9,000 3 View
Hello, I am working on production and purification of functional amyloids, that are also involved in biofilm formation. The thing is that to identify amyloid producing strains I would like to have...
02 November 2021 4,776 0 View
Dozens of experiments have been performed with light rays, beginning from Fizeau, in order to establish the local constancy of the speed of light and verify how speeds sum up. It was verified...
25 October 2021 5,193 99 View
A spin trapping agent such as DMPO can trap radicals for the formation of radical adducts. However, many other impact factors including cations/anions, molecules, redox conditions could influence...
30 September 2021 3,376 1 View
Hi, I need a power system model with 2 - bus, 2 - machines and a long-distance HVAC transmission line. Would you mind providing with me all the necessary parameters of the system? Bergeron line...
29 September 2021 9,367 6 View
i am looking for the time dependence of hummers method, how long should i proceed the reaction before quenching with h2o2
27 September 2021 8,337 2 View
I added jaggery and fermented it in water. I would like to know the quantification of alcohol. What are the methods that can be used for the quantification?
21 September 2021 877 2 View
I use 3T3-L1 fat cells. After it differentiated into mature adipocytes, I first starved the cells with serum-free starvation medium for two hours, and then stimulated the cells with insulin at...
11 September 2021 274 2 View
We have found some evidence for behavioral habituation to repeated muscimol microinjections into the orbitofrontal cortex during an aversive operant procedural task. The effect seems to wear off...
09 September 2021 2,390 2 View
2-aminoterephthalic acid (H2ATA) (0.5 g, 3 mmol) and tetra-n-butyl titanate Ti(OC4H9)4 (0.26 ml, 0.75 mmol) were added into a solution containing dimethylformamide (DMF) (9 ml) and dry MeOH (1...
24 August 2021 8,606 2 View
I would like to photoactivate, via laser, a protein with GFP tagged to it (inside a solution) and then visualize the activated GFP's fluorescence on a non-denaturing polyacrylamide gel. An...
11 August 2021 3,730 2 View