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Questions related to Dyes
I am working on the electronic band structure of some topological insulators. When I doped some impurity into it, the valence band of it moved downward away from the Fermi level while the...
05 June 2024 6,253 3 View
Hello, I have propagated a plasmid form a single colony (size around 15 kb) using small scale culture (2 ml of TB with selected Antibiotic) and purified it using Mini-prep kits ( FastGene Plasmid...
15 May 2024 6,111 2 View
Hello ~ I'm currently beginner using Wannier90. My target molecule is "EuTiO3". The electron configuration of this molecule is as follows: Eu: 1s2 2s2 2p6 3s2 3p6 3d10 4s2 4p6 4d10 5s2 5p6...
07 May 2024 2,037 1 View
I'm performing some DAB-enhanced anti-GFAP staining. My primary is polyclonal chicken anti-GFAP (AB5541): The antibody isotype is described as N/A in the SDS. We have a goat anti-chicken IgY...
15 April 2024 9,703 1 View
Dear high-qualified expert biologists, The likelihood of rejection of 3D bioprinted of endocrine glands, e.g. by Roll Porous Scaffold technology, made from a person’s own DNA is minimal. Thanks...
18 March 2024 3,537 0 View
I and using vectors with autoinducibe promoters(Pylb and cyt1). I have performed SDS of 24, 48, 72, 96 and 120 hours. No expression is found in soluble or insoluble form.
15 March 2024 8,741 0 View
How do I measure the uptake of BPA in soybean plants treated with a BPA solution? I have an HPLC, NMR, UV-Vis, GC, XRD.
29 February 2024 7,532 0 View
Why is Fermi level closer to conduction band in n-type semiconductor and relationship between Fermi level and band structure for n-type semiconductor?
29 February 2024 3,125 0 View
Among the articles I am reading, there is a phrase that says "Bi3+ has a lone pair of electrons in its 6 s subatomic shell, which is responsible for the observable defect screening ability." But I...
29 February 2024 2,392 0 View
the MOF says about the Metal organic Framework
06 February 2024 5,983 0 View
What specific herbal remedies have shown promising results in preventing or treating urinary stones, and what is the recommended approach for incorporating phytotherapy into a comprehensive...
20 January 2024 8,336 2 View
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15 January 2024 2,928 3 View
Hello all! I am currently working with the single-molecule force spectroscopy using AFM (Multimode scanning probe microscope with a Nanoscope V controller (Bruker Inc.)) under constant mode to...
15 January 2024 1,335 0 View
Hi everyone, I run into a bit of trouble while making a loading dye for my PCR using Bromophenol Blue. The recipe I used is 25 mg of Bromophenol Blue + 3,3 mL Glycerol + 6,7 mL H20, but my...
11 January 2024 4,525 0 View
I want to study the viscosity of the of ferrofluids. Would anyone please suggest me institutes in India where I can do magneto rheology studies ?
11 January 2024 2,964 1 View
How microorganisms are important for maintaining of health soil and biodiversity and role does soil play in the cycling of energy and nutrients?
09 January 2024 8,319 4 View
I acquired a DNA Analyzer 3700 from a third-party source approximately one year ago. Regrettably, I have encountered difficulties utilizing the equipment as the accompanying software appears to be...
03 January 2024 9,557 0 View
What are the ecological roles of microorganisms and role of micro organisms in the decomposition of organic matter and recycling of carbon?
03 January 2024 4,150 3 View
Where can I find pigmented short hair guinea pigs for laboratory? Do you know any sellers?
27 December 2023 7,908 2 View
Hello everyone, I'm curently working on HaCaT cells which require low calcium medium to return to undifferentiate state. To do so, I need to chelate my FBS for 1h with chelex resin among other...
13 December 2023 4,959 2 View
Problem After Transferring the membrane picture with Ponceau S Staining looks like the membrane is burning and has poor transfer. Can someone help with this? I also noticed something weird on...
12 December 2023 9,946 2 View
Dear Researcher, please help me to calculate the BET surface area and pore size from BET analysis results. Also assist me in drawing and interpreting isotherms.if there is any research article or...
07 December 2023 1,160 4 View
When i synthesize the perovskite nanocrystal, after centrifugation, there are a PL peak at 500 nm and a shoulder at around 440nm, which should belong to nanoplatelets. i don't want to this...
04 December 2023 6,181 2 View
Although the donut concept of the optic disc is widely published in glaucoma but it is not supported by any histology in the textbooks.
03 December 2023 1,682 2 View