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Questions related to Dyes
What role do microbes play in the cycling of carbon and their possible influence on global warming and role of microbes in environmental conservation?
30 March 2023 1,219 3 View
Hello. I plan to experiment using mouse brains with fluorescence in situ hybridization (FISH). In particular, I want to measure the expression level of some genes in specific neurons labeled with...
24 March 2023 3,248 0 View
i´m triying to design a expression cassette for a monoclonal antibody, that needs to be produce in a tomato plant. i have a previous aminoacid sequence of the gene i need, from a previous work...
22 March 2023 6,145 3 View
I am a second year student at Bangor University looking for a dissertation question for my final year. I am mainly interested in working in the field, mainly basing my research on herpetology...
21 March 2023 3,495 2 View
Good Day Recently in my AP recordings, I often observe that there is a time delay in my current injection recordings. For example, if I set the protocol to inject at 1s, the actual recording...
19 March 2023 2,275 0 View
I'd like to reduce COD in waste that contain organic amine. I've tried to add hydrogen peroxide (no catalyst addition) but, the final COD result was higher than the initial. Maybe there's a...
17 March 2023 335 5 View
Dear everyone, I want to do some calculations about NH2-MIL(Cr)-101, but I cannot obatain its structure file, could you please give me some suggestions?
17 March 2023 2,956 0 View
I used a Domiphen Bromide in the concertation of 0.025 w/v as preservative in the oral liquid formulation. Its effectiveness has been proven by the Antimicrobial Efficacy Test. Is it used...
09 March 2023 5,681 3 View
How to perform FeFET simulation in TCAD (Silvaco ATLAS)?
07 March 2023 5,888 1 View
I have synthesized carbon coated material. however when I performed TGA, there is weight gain in my bare sample and weight loss in carbon coated sample?
02 March 2023 9,179 3 View
Fabricating PEG and SA blended membranes with Polysulfone.
01 March 2023 3,886 0 View
frog
28 February 2023 3,914 6 View
Dear wise person, I have recently conducted TMT proteomics of cultured cells. I am unsure about how certain the detection is. Do I understand it correct mass spec cannot distinguish between...
16 February 2023 9,213 2 View
For example, I have a single mode fibre based Mach-Zehnder interferometer, when I put it into a temperature stablised enviroment, there would be a linear phase drift? What is the phase drift at...
15 February 2023 871 3 View
As the question already implies, i made a mistake during restriction enzyme digestion and added the 6x loading dye to the DNA insert fot checking on the gel. 10ul was added to the gel for checking...
15 February 2023 6,037 2 View
I want to use PPS (Polyphenylene sulfide) cloth instead of PTFE (Polytetraflouroethylene) cloth filter in a filtration process, is there any limit to this practice? What is the main difference...
12 February 2023 7,313 4 View
Am I right that isi is caused by multipath fading and that should pass through same zero crossing ??
08 February 2023 1,471 2 View
There is an approach that has been put forward recently for this purpose known as DNA. There are many doubts about the feasibility and effectiveness of this method. How reliable is it and is there...
31 January 2023 9,290 1 View
I’m running a batch of mouse tissue tagged with TMT-16plex. Samples were fractionated in Agilent after S-trap. They look really clean… All maintenance was done in nLC. Precolumn and analytical...
14 January 2023 1,278 0 View
I have images stained with different immunofluorescent markers (DAPI + many different markers) and captured images. However I recently discovered when I went to Metamorph to compare that the...
13 January 2023 6,950 1 View
Silver nanoparticles can be positively, negatively or neutrally charged depending on the method of synthesis. However, I used commercial silver nitrate. How can I know the charge of silver...
25 December 2022 1,231 4 View
Background Info to experimental set-up: Mutated Mucin-1 is found to be aberrantly expressed in diverse cancers and has been detected in certain mature B-cell malignancies such as Multiple Myeloma...
24 December 2022 5,501 0 View
I performed an RT-PCR on my gene of interest hoping to see which isoforms are present on the mRNA level. Literature suggested there should be 4. To my surprise, i see lots (too many to count) of...
23 December 2022 6,536 1 View
I am doing TEM on a rare cell population (10k cells) - I am aware of methods for processing such small samples for TEM via agar embedding the pellet and using Evans blue to visualize the pellet...
22 December 2022 7,360 0 View