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Questions related to Dyes
I am working on DOPC lipids and I want to characterise them under epifluorescence microscope? I‘d like to know what concentration of 3 3'-dioctadecyloxacarbocyanine perchlorate dye would be the...
14 November 2020 9,390 3 View
I also want to use Spectro fluorometer to measure calcium concentration. One more thing, after treating cells with calcium dye, Should I lyse the cells or not to measure calcium concentration?...
12 November 2020 7,891 3 View
I'm using puromycin for selection, usually I'm making 50 ml of growth media that lasts for about a week, I'm wondering if Its possible to make 500ml and have the puro remain stable for a whole month.
09 November 2020 6,123 0 View
Hello everyone. I`m getting a good amplification in the LAMP reaction (see figures), but I have received no change in color of pH-sensitive dyes (f.c. 0.05-0.1 mM of the Phenol Red, Cresol Red,...
30 October 2020 1,359 3 View
Hello, I would like to know the mechanism behind why "membrane impermeable dyes" are not able to penetrate through the phospholipid cell membrane. Also, does this mean they are also impermeable...
29 October 2020 3,059 3 View
Hi everyone, I made a mistake when I computed how much antibody I needed for my FACs, so I have an excess of exactly 10 reactions. Since they are so expensive and our lab is so poor, I was...
28 October 2020 3,662 3 View
I have sperm cells extracted from epididymis of a mouse. I would like to check and quantify their purity. My first idea is to use an intercalating dye like Hoechst and then see what fraction of...
26 October 2020 8,782 1 View
are there studies (dyes or confocal laser scanning microscopy) on depth of penetration of citric acid into dentinal tubules?
13 October 2020 9,016 1 View
Hi yall, We are currently working on rhodamine involved research project. Does anyone know how rhodamine molecules give EPR signals? I mean the mechanism behind the unpaired electron formation of...
13 October 2020 5,505 2 View
We use dye as a model compound in photocatalytic experiments. How effective the process? The color of dye can hinders the photons reaching the catalytic surface. Would like to know any work on...
11 October 2020 3,763 2 View
I am currently analyzing the composition of an unknown solution. It is a blue dye solution, but there are an awful lot of blue dyes. I have already analyzed the UV-VIS spectrum, the wavelength is...
09 October 2020 4,771 4 View
I want to know the concentration of a home-made saturated aqueous solution of Bromophenol blue. How could I know when I add unknown number of grams of the dye?
06 October 2020 3,689 3 View
On the website of the company Biotium that sells them they seem great, of course. But can anyone provide practical experience comparing say, CF 488A to similar dyes like Alexa 488 and Dylight 488...
05 October 2020 565 3 View
I cannot find any supplier in Europe of this dye. Than you !
05 October 2020 9,419 2 View
i am studying the variables (study of initial dye concentration ) for removing methylene blue (mb) using a composite that i prepared. the results showed that on increasing dye conc, the removal...
02 October 2020 3,164 3 View
Hi, I need to dye bivalves to bring out the details of the shell and to be able to take good photos. I have heard that there is a gaseous compound that allows this and doesn't damage the samples,...
28 September 2020 2,035 4 View
what is the permissible limit of leather industrial dye? I will try to search the paper related to this topic but don't found.
21 September 2020 6,402 3 View
Hi all, I was wondering what is the best way to get rid of the organic dye off my mini-gelfiltration column. I washed my column with excess 20X H2O and the dye still seem to be there....
18 September 2020 9,980 2 View
Hello..please i did run MD simulations for 50 ns and the results i had for my radius of gyration doesn't look good. can someone please tell me where the problem is coming from and how to solve...
12 September 2020 4,699 0 View
Hi everyone, I am studying on the adsorption of methylene blue using inorganic based adsorbents. As a result of my study, although the amount of adsorbed dye is approximately 95%, I found that the...
10 September 2020 7,782 4 View
We are analyzing T cells in response to X antigen, and it seems there is an authoflourescent after compensation has been done. I was told that in order to elilminate autofluorescence i have to...
10 September 2020 5,306 4 View
When to add ROS generation binding dye H2DCFDA, before or after treatment and why?
08 September 2020 5,128 3 View
Hi! I was wondering if CF488 Maleimide dye from Sigma (https://www.sigmaaldrich.com/catalog/product/sigma/scj4600016?lang=de®ion=DE) penetrates the cell walls of live Vibrio cholerae. All I...
27 August 2020 1,101 2 View
I am working on a Luminescent Solar Concentrators (LSC) using dyes such as Coumarin 1, Coumarin 153, and Perylene Green Ph-probe 720-5.5. I need to calculate the Mean-free-path (MFP) for the...
19 August 2020 9,964 3 View