How to run a mediation analysis with two different samples?

Hi, I tried to run a mediation analysis in which the DV is made up of test scores after a training in the experimental group VS the control group, and in which the mediator is measured by a...

17 November 2020 7,178 3 View

How can I add a chain identifier to a PDB file generated by Phyre2?

I am trying to use DynaMut2 to study stabilizing/destabilizing effects of mutations in a protein. I generated a 3D structure using Phyre2, and it gave me a PDB file without a chain identifier....

22 September 2020 495 11 View

Survey data collection sample purchase - Qualtrics or Dynata?

I am planning for a survey research project (national representative sample for 15 min survey) and am considering which panel/sampling company to use. I have used Dynata before, they are...

22 September 2020 9,334 4 View

Protocol for using Attune NxT Acoustic Focusing Cytometer to detect Ploidy level in leaf tissue?

Hello, I am looking for a detailed protocol to use the Attune NxT Acoustic Flow cytometer to determine ploidy level in plant samples with PI staining. Details for laser, threshold, and...

21 September 2020 8,334 2 View

Can a study have multiple research philosophies ?

I would like to know whether a study can have multiple research philosophy. I am currently studying the influence of ownership on firm performance through regression analysis. I am using case...

19 April 2020 1,073 3 View

How do you define the ontology and epistemology of mixed methods research?

The research which i am doing consists of multiple regression and case studies research. I would like to know whether what i am doing is a mixed methods. Also, what are its ontological and...

08 April 2020 5,457 15 View

Somebody know a fast DNA extraction method for plant for to do in the field?

I am looking found reference about people that has been working the proposed method in this publication: "Nucleic acid purification from plants, animals and microbes in under 30 seconds" from Zou...

10 March 2020 860 2 View

Is there any relevant literature detailing extraction methods of simple sugars from plant material for LC-MS analysis?

For my research, I would like to extract glucose, sucrose, and fructose from the crowns of strawberry plants and quantify these sugars using LC-MS. However, I have not had much luck finding a...

11 February 2020 398 3 View

How do I isolate LC nucleus for cell culture?

I need to isolate LC for primary cell culture? How should I do? What is better: use frozen tissue or fresh tissue?

21 January 2019 9,383 3 View

What happen if I put enzyme on optimum pH and temperature but I did not put any substrate to digest / hydrolyse?

I'm doing BSA test and I need a blank to identify the product of my substrate (which means I can't use water since BSA will read my enzyme as protein as well) ; problem is, once I substract the...

07 November 2018 5,006 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can COVID-19 vaccines protect against the emerging strains of SARS-CoV-2?

Can COVID-19 vaccines protect against the emerging strains of SARS-CoV-2?

18 February 2021 8,221 8 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

What are the popular techniques for pre-processing measurement data in Smart Grid/Power System?

The data collected from various measurement devices often contains erroneous values - such as noise, missing values, etc. What are the popular techniques for pre-processing measurement data in...

14 February 2021 4,642 2 View

How to improve the adhesion of epoxy coating on steel substrate? Which factor affects?

I am facing a problem regarding a de-adhesion problem with the coating. A brief description of my methodology is given below. I am using mild or Low Carbon steel as a substrate and I polish the...

14 February 2021 2,167 4 View

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

11 February 2021 6,628 9 View

Can explain this diagram to me, is this the average consumption curve for one day or the total consumption of days in a month summed up in specific?

The hourly load curves of the selected household in each season are shown in Fig. 28. Figure 28 shows the demand for electricity in four months on an hourly basis. Can you explain this diagram to...

08 February 2021 657 16 View