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Questions related to Dyes
I’m running a batch of mouse tissue tagged with TMT-16plex. Samples were fractionated in Agilent after S-trap. They look really clean… All maintenance was done in nLC. Precolumn and analytical...
14 January 2023 1,453 0 View
I have images stained with different immunofluorescent markers (DAPI + many different markers) and captured images. However I recently discovered when I went to Metamorph to compare that the...
13 January 2023 7,103 1 View
Currently, we only know of testing ink toxicity by identifying the presence and concentration of metals, metal oxides, and polymers through Gas Chromatography-Mass Spectrometry (GCMS) and X-ray...
29 December 2022 8,260 1 View
Silver nanoparticles can be positively, negatively or neutrally charged depending on the method of synthesis. However, I used commercial silver nitrate. How can I know the charge of silver...
25 December 2022 1,423 4 View
Background Info to experimental set-up: Mutated Mucin-1 is found to be aberrantly expressed in diverse cancers and has been detected in certain mature B-cell malignancies such as Multiple Myeloma...
24 December 2022 5,673 0 View
I performed an RT-PCR on my gene of interest hoping to see which isoforms are present on the mRNA level. Literature suggested there should be 4. To my surprise, i see lots (too many to count) of...
23 December 2022 6,659 1 View
I am doing TEM on a rare cell population (10k cells) - I am aware of methods for processing such small samples for TEM via agar embedding the pellet and using Evans blue to visualize the pellet...
22 December 2022 7,489 0 View
Hello, I have a vector for expressing a protein tagged with EGFP in the c-terminal and I want to remove the eGFP by adding a stop codon, how can I do that? Thank you
19 December 2022 2,607 4 View
I run a qPCR (Taqman probe) to detect shiga-toxin gene (stx1) with rotar gene Q (qiagen) thermal cycler. My primer & probe are - F - 5'-TTTGTYACTGTSACAGCWGAAGCYTTACG-3' Base - 29, GC - 44.8%,...
18 December 2022 3,119 3 View
Preferably data on potassium loss from maize cultivation of mineral acid soils would be useful.
16 December 2022 478 1 View
Does anyone have tips or advices to disminishe photobleaching of PRODAN dye on fresh tissues? i'm currently running some experiment in plant tissues with this dye and i want to prolongue the...
06 December 2022 5,776 0 View
Hello all, I am in search of a process to doubly label the proteins with different fluorophores at different positions in the same protein. Say for instance, If I conjugate dyeA at N-terminal...
02 December 2022 3,985 5 View
I followed the degradation of dye till 24 hour .. so how I should calculate rate constant because i take UV with a gape of 1 hour. what its unit will be?
30 November 2022 4,221 2 View
An EPR spectrum of an organic compound was recorded on solid state which resonated at around 83 mT but on calculating the g-factor the value came out be nearly 8.00 which is too high than...
28 November 2022 8,404 4 View
Recently, I made a 20% glycerol solution, added red ink to it, and placed a drop at my microfluidic chip's inlet. But it needs to move forward, and it is not moving forward. What's the reason...
25 November 2022 5,969 6 View
Dear Sir, this is to kindly inquiry a material on tropical agronomy & crop response to environment in the forms of lecture note, text book, article or any forms of document. with kind regards,
23 November 2022 7,246 3 View
I want to do free protein MD simulation of the protein pdb I'd 3LV3, that's why I'm telling if any inhibitor part is attached to the free protein or not. If so, on which chain the inhibitor part...
22 November 2022 2,596 1 View
I have the dot plot and the histograms of the samples after the treatment with the fluorescence-labeled nanoparticles.
21 November 2022 1,386 0 View
I've started working on a Project that involves staining actin filaments with Phalloidin-type dyes. Currently we're using Phalloidin-Atto 488, however the photobleaching happens quite quickly,...
17 November 2022 2,102 0 View
Hello, I have a question on TMT labeling MS. Why is it that we randomize our samples (we randomize with an excel function) for TMT labeling? Much appreciated, Andrew
16 November 2022 3,983 0 View
Hello guys, I performed an experiment using Mitotracker Deep Red FM from Invitrogen and analyzed it by flow cytometry. In this experiment I used two lineage of cells, being one of them knockout...
14 November 2022 7,862 2 View
Organic and inorganic contaminants removal experiments have been performed at pH? How the of the solution is kept constant? Are disturbing ions not effective in the removal process?
14 November 2022 2,733 2 View
I see varying sources describing this dye. One place says it binds via its hydrophilic groups. Another says its a lipophilic dye. Which is it, how does it bind to internal membrane structures...
13 November 2022 5,263 1 View
I am going to write an overview article and unfortunately, I am not an elegant expert. Its my pleasure, anybody can help me.
06 November 2022 3,837 5 View