Contact experts in Dyes to get answers
1,364 views 1,540 posts
Questions related to Dyes
Hello, I've got several VHH sequences after phage pannings and cloned them into pET28a vector to express. After transforming them into BL21(DE3) E.coli strain and inducing with IPTG, I found...
14 March 2021 8,973 7 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
02 March 2021 9,700 3 View
Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...
20 February 2021 686 5 View
Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...
05 February 2021 2,487 3 View
Hi I have a protein labeled with rhodamine B and after reaction i used Zeba spin column for dye removal. my problem is that lot of unreacted dye remained which interfered with my florescent...
03 February 2021 814 2 View
I am using methyl violet 10B and reactive orange 16 (RO16) dyes for my Ph.D. research. I'm trying to find the molecular size for both of these dyes. It is possible for me to find these dyes'...
02 February 2021 3,515 4 View
For Example: If we take the botanical (Amra Pods) and we are using the dyes to give color to Amra pods . Here I take Acrylic Red(Dye). Im using 100% dye to amra pods to get it colored. Is there...
01 February 2021 1,189 1 View
In our experiment, the top of our ladder indicates 12,000bp, and in subsequent lanes we have samples where the band shows up slightly above the top of the ladder (however we know for a fact that...
28 January 2021 5,878 3 View
Hi guys, I'm looking for a water-soluble dye which is not surface inactive in order to observe (water in oil) emulsions; I don't prefer to use oil-soluble dyes. I've used rhodamin123,...
22 January 2021 8,657 2 View
Hi! I am wondering if someone can recommend me a good dye for measuring mitochondrial potential. I have used TMRE with good results in the past, but I have heard also about Mitoview. I would use...
13 January 2021 4,316 4 View
I have make mix of mortar with ordinary Portland cement and standard sand, according ASTM C109 the mix ratio of cement:sand:water was (1:2.75:0.485) but the flow value was very low (about 24%)...
13 January 2021 6,074 23 View
I mean I suppose it must be removed somehow, otherwise metabolic processes within the cell would continue and the cell would not be preserved. But, even under vacuum during the process, the...
06 January 2021 4,684 4 View
i'm working in Counter electrode. The device architecture is TiO2
29 December 2020 8,032 4 View
A solution having 2 compounds in it whose degradation is measured by checking absorbance. How can we measure absorbance of such a solution when lambda max is different for these 2 compounds?
25 December 2020 2,254 3 View
I want to calculate the absorption spectrum of 3 dyes malachite green, brilliant green and ethyl green through a GAUSSIAN 09 calculation but I cannot find results close to the experimental values...
20 December 2020 5,536 3 View
I am running qPCR on eDNA samples from rivers and have found that the vast majority of my samples show a decrease in fluorescence in my passive reference dye (ROX) while my target dye (FAM)...
16 December 2020 890 4 View
Dear all, I'm searching for methods to calculate Forster Radii of FRET pairs, ideally for water-soluble and membrane dyes. Although I am relatively familiar with working with microscopy FRET, I am...
08 December 2020 3,225 3 View
Hi everyone, I am a student trying to quantify the number of cells in an image using ImageJ. Is there a way to bring all my images from a set of experiments to a set intensity? For example, if...
06 December 2020 7,268 2 View
I'm working on the degradation of various pollutants with bare and composite TiO2. Since the size of TiO2 is too small, the particles won't be settling down as we take the dye solution at...
02 December 2020 2,220 8 View
Please suggest validation test to confirm in the photocatalytic reaction.
25 November 2020 1,007 3 View
Between depth of colour (K/S), DE_cmc and invidual L*CH (lightness, chroma, hue) parameters of a dyed fabric, which one would give best estimate of whether one sample has greater/lower dye...
23 November 2020 1,056 2 View
Hello! I would like to test the expression of some intracellular cytokines in primary T lymphocytes by FACS. I need to test the effect of a molecule on their expression. I found a lot of...
19 November 2020 3,628 5 View
We would like to electroporate neurons in organotypic slice cultures with DNA for genetically-encoded flourescent reporters (pH reporters, GEVIs etc). We would like to use an anionic dye that can...
18 November 2020 2,880 1 View
To remove the pollutants from textile wastewater using electrocoagulation, the targeted pollutants are Color, turbidity, and COD. Thus, to get the optimum operating parameters first, we planned to...
15 November 2020 8,714 6 View