Contact experts in Dyes to get answers
1,378 views 1,540 posts
Questions related to Dyes
As both Chlorophyll semi-synthetic derivative and methylene blue organic dye has been widely used as a potential photosensitizer in applications like PDT but I want to know that; a chlorophyll...
16 August 2020 6,450 3 View
I need a fluorescent dye for my system at pH -9. Specific requirements are hydrophilic in nature and neutral. I don't wanna use the dye loaded micelles as it interferes with the experimental...
06 August 2020 10,069 3 View
Hello, I'm running a research on wild mammals with camera traps (capture mode: photo).I 'd like to analyse the data using tracking software to get information about animals activity (time spent...
28 July 2020 5,438 1 View
for microbiological media preparation, methylene blue is added before or after autoclave????
25 July 2020 8,878 1 View
For covid19 diagnosis TaqPath kit is having ABY n JUN dye... But in our lab Biorad Cx96 machine is available where ABY n JUN dye are not available. How can we use the Biorad machine for covid19...
23 July 2020 2,194 2 View
Hi. When I purify my His-tagged membrane protein with IMAC, I label the protein (containing a single cysteine) with fluorescein-5-maleimide while the protein is immobilized to the Ni2+ NTA. I...
21 July 2020 2,207 3 View
I am trying to quantify the Plasma Membrane cholesterol, total cellular cholesterol as well as the the amount of Cholesterol esters inside the cell by Amplex Red Cholesterol Assay Kit. I have also...
19 July 2020 5,820 0 View
Does anyone have a suggestion for a method to florescently tag some dyes to the cell lysate proteins? I am searching for a bioconjugation method that can label most of the proteins in the lysate....
11 July 2020 9,173 2 View
Hello, I am looking to calculate the most accurate data for the cations, neutral molecule, and anions of carbon monoxide. At this point I have some preliminary data using empirical and ab inito...
11 July 2020 5,586 0 View
Can anyone suggest a protocol (with reference) on how to prepare the exosomes for CONFOCAL STAINING? Details- exosmes will be isolated from culture media at 100,000g and will be resuspended in...
01 July 2020 6,400 2 View
In order to prove a reaction mechanism using the Fukui function, some intermediates are originally anions or cations as in the case of steglich esterification (see photo attached). the question is...
22 June 2020 9,976 2 View
I have conjugated a dye to a goat anti-rabbit secondary antibody. I believe that the more dyes that I conjugate to the antibody, it then loses its ability to recognize the primary antibody. I...
19 June 2020 4,754 3 View
How ultrasound and microwave can be effective factors on cotton and wool fabric which help the fabric to improve its dye uptake ability?
16 June 2020 4,638 3 View
I prepared polydopamine layers with silver nanoparticles and tested for SERS. SESR intensity of the lowest rhodamine B solution concentration (10-7 mol/l ) is higher than the one of the most...
14 June 2020 7,944 3 View
I am new to calcium analysis and I have to analyse calcium imaging data of human induced pluripotent stem cell derived cardiomyocytes. Does anyone know any good tutorials/papers or have any...
11 June 2020 4,554 3 View
I am doing dye removal experiment and need to calculate Qmax value from Langmuir Equation, Kindly help me with example .
09 June 2020 9,136 1 View
From above, it is normal 13 carbon nmr, dept135, dept90, dept45. So...4 peaks in 0 to 40 ppm is negative peak at dept135. That means, those 4 peaks is -CH2-. But I can see two peaks in 0 to 40 ppm...
08 June 2020 5,892 0 View
Dear all, I want to study change of microbial species and community structure caused by some outside factors like dye and heavy metal in room. Can I just take soil of specific place and put them...
03 June 2020 4,266 5 View
Dear all, I'm currently using a commercial AO/PI dye to count live T cells, which is not cheap. Since AO and PI is available and cheap, I'm wondering if anyone can provide me a protocol to make...
21 May 2020 7,258 0 View
I need to know more about N719 like its excitation wavelength, its band gap and all features in related to conductive characteristic.
16 May 2020 8,828 0 View
I am preparing waste water with congo red dye but the dye is converting into violet colour after few hours. The composotion of waste water in (mg/L)- NH4NO3-176.1, NaCl- 7.0, MgCl2.6H2O- 3.4,...
08 May 2020 8,791 0 View
In DSSC, why electron cannot directly transfer from LUMO of dye to conduction band of electrolyte? I wonder that the conduction band of electrolyte is also lower than LUMO of dye, but electrons...
04 May 2020 3,629 0 View
Is there no need to labell the dye with the primer ? Master Mix given by Edgardo E Salvatierra Mix Conc. ul h2o 15.15 Buffer 10 X 2.5 MgCl 50 mM 1.5 dNTPs 10 mM 0.5 Primers 5 uM 2 BSA 10 mg/ml...
22 April 2020 8,117 0 View
In terms of tagging/immunofluorescence/radiolabelling
20 April 2020 9,479 0 View