Contact experts in Dyes to get answers
1,375 views 1,540 posts
Questions related to Dyes
Hello, I have a vector for expressing a protein tagged with EGFP in the c-terminal and I want to remove the eGFP by adding a stop codon, how can I do that? Thank you
19 December 2022 2,423 4 View
I run a qPCR (Taqman probe) to detect shiga-toxin gene (stx1) with rotar gene Q (qiagen) thermal cycler. My primer & probe are - F - 5'-TTTGTYACTGTSACAGCWGAAGCYTTACG-3' Base - 29, GC - 44.8%,...
18 December 2022 2,939 3 View
Preferably data on potassium loss from maize cultivation of mineral acid soils would be useful.
16 December 2022 360 1 View
Does anyone have tips or advices to disminishe photobleaching of PRODAN dye on fresh tissues? i'm currently running some experiment in plant tissues with this dye and i want to prolongue the...
06 December 2022 5,684 0 View
Hello all, I am in search of a process to doubly label the proteins with different fluorophores at different positions in the same protein. Say for instance, If I conjugate dyeA at N-terminal...
02 December 2022 3,857 5 View
Dear Sir, this is to kindly inquiry a material on tropical agronomy & crop response to environment in the forms of lecture note, text book, article or any forms of document. with kind regards,
23 November 2022 7,087 3 View
I have the dot plot and the histograms of the samples after the treatment with the fluorescence-labeled nanoparticles.
21 November 2022 1,226 0 View
Hello, I have a question on TMT labeling MS. Why is it that we randomize our samples (we randomize with an excel function) for TMT labeling? Much appreciated, Andrew
16 November 2022 3,820 0 View
Hello guys, I performed an experiment using Mitotracker Deep Red FM from Invitrogen and analyzed it by flow cytometry. In this experiment I used two lineage of cells, being one of them knockout...
14 November 2022 7,703 2 View
Organic and inorganic contaminants removal experiments have been performed at pH? How the of the solution is kept constant? Are disturbing ions not effective in the removal process?
14 November 2022 2,594 2 View
I see varying sources describing this dye. One place says it binds via its hydrophilic groups. Another says its a lipophilic dye. Which is it, how does it bind to internal membrane structures...
13 November 2022 5,151 1 View
I am going to write an overview article and unfortunately, I am not an elegant expert. Its my pleasure, anybody can help me.
06 November 2022 3,707 5 View
Hi everyone, Is anyone here familiar with the CUPRAC method? I am struggling with creating the standard curve of that method. My procedure is the following: 500ul PBS 1X + 500ul CuSO4 1M + 500ul...
02 November 2022 7,667 3 View
This is a gel with the trnH–psbA marker. Gradient PCR 54-57°C for a Nepeta species collected from nature (upper lanes) and a Centaurium species from in vitro conditions (lower lanes). We're...
25 October 2022 3,378 1 View
I am working in the area of organo-bentonite. After the organic modification of bentonite, some changes in the PXRD patterns of bentonite observed that could be due to layer exfoliation. Moreover,...
24 October 2022 6,870 2 View
I received in my e-mail the text: "Your Paper Impact Effectiveness (PIE) Value: 44.09." "Dear Researcher, Considering the total number of citations as the sole indicator of a research paper’s...
23 October 2022 6,225 2 View
I made working electrode of bare TiO2 film, carbon quantum dots (CQD) coated TiO2 film, N3 dye loaded TiO2 film, and CQD+N3 dye coated TiO2 film. FTO glass (7 ohm/sq) was used as substrate. Pt...
21 October 2022 8,753 2 View
What can I use to preserve insects? will 70% work? or I can use 90%. The same goes with plants, what can I use to maintain them before I conduct molecular analysis? The specimens must be...
18 October 2022 3,516 4 View
Hello please help in finding the area under the curve (as in the attached figure) in R. The curve has been created using logspline function on histogram (density )
13 October 2022 3,746 3 View
What is a reliable source for absorption spectra of free fatty acids? I have absorption spectra for multiple free fatty acids that I'd like to compare with. I've only been able to locate a few...
12 October 2022 3,373 1 View
Dear researchers, Considering the dimensions of the Plexiglass DCMD cell, I want to fabricate a VMD cell only by removing the cold water inlet. Then we have one inlet (hot water) and two...
11 October 2022 2,159 0 View
Hello, my gaussian 09 calculations are stopping in SCF cycles and the is given the following message "traceback is not available". Did one help to solve this? - End of the output file: Cycle...
28 September 2022 1,234 1 View
We used to use the retrobeads to mark the injection site and it did work well. But we can't use them in the current experiment, as we will perform sequencing afterwards and also cannot allow any...
02 September 2022 9,622 0 View
I am trying to measure Calcium influx in TRPV4 stimulated/unstimulated RAW 264.7 cells using Fura-2 AM (incubation for 45mins at 37degrees) and a plate reader. I was hoping that when I get high...
14 August 2022 2,248 6 View