As the question already implies, i made a mistake during restriction enzyme digestion and added the 6x loading dye to the DNA insert fot checking on the gel. 10ul was added to the gel for checking but the other 60ul that was supposed to be used for ligation later on also has the 6x loading dye added to a concentration of 1x. Will this be a problem later? Will the dye be removed during the GeneJet PCR purifications later? How should i proceed? All input is welcome.
Kind regards.
You can purify the digested product using a PCR cleanup kit. It would remove all the dye & buffer and you would have only digested product.
Hi there,
As when you perform gel purification, the silica column purification process allows to remove the DNA "staining" molecules...
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