I am doing TEM on a rare cell population (10k cells) - I am aware of methods for processing such small samples for TEM via agar embedding the pellet and using Evans blue to visualize the pellet embedded in agar.
Can this same protocol of agar embedding and staining with Evans blue be used for immunogold? will the Evans blue ruin the antigenicity of the sample in the pellet? I am at a loss as to how to process these pellets otherwise as they are impossible to see.
Osmication would obviously greatly aid in visualizing the cell pellet however I cannot use OsO4 for immunogold applications.
Are there any other stains that I could potentially use to visualize the pellet while still maintaining antigenicity? Any advice would be very appreciated.
Thank you.
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