I see varying sources describing this dye.
One place says it binds via its hydrophilic groups.
Another says its a lipophilic dye.
Which is it, how does it bind to internal membrane structures like mitochondrial and golgi membranes?
Hello Rh Rj
DiOC6 is a lipophilic cationic fluorochrome that is membrane permeable. It is used for labeling membranes and other hydrophobic structures. Once applied to cells, the dye diffuses laterally within the cellular plasma membranes, resulting in even staining of the entire cell at its optimal concentration.
It is selective for the mitochondria of live cells, when used at low concentration. At higher concentration, the dye may be used to stain other internal membranes, such as the endoplasmic reticulum and the Golgi apparatus. Binding to these structures occurs mainly via the dye's hydrophilic groups.
Because of its cationic nature, it is a membrane potential-sensitive dye that can be used to probe mitochondrial membrane potential according to the accumulation of DiOC6 dye in the mitochondrial matrix.
Best.
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