How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Can Scree Plot in SPSS show an incorrect data on X-axis?

Hi! I'm working on a PCA in SPSS 23. I have 78 items. PCA extracts 16 components. However, scree diagram is plotted with items (manifest variables) on the X-axis. Not components (factors). See...

27 January 2021 5,919 3 View

How to measure Tg (Glass Transition) in lyophilised sugar/protein matrices using DSC?

I have been attempting to measure the glass transition temperature of various amorphous powders consisting of sugar/protein mixtures and have had little luck. I typically use a scanning rate of...

13 January 2021 10,022 3 View

Where to publish my methodological working paper?

Recently I decided to switch my PhD project to a cumulative form. I have already written an extensive methodological chapter about my adaptation of interpretivist methodology and ethnographical...

11 January 2021 5,337 6 View

Why does reducing my protein samples with TCEP cause the emergence of a new, much lower MW band in SDS PAGE?

Hi! I study the effect of formulation excipients on protein degradation pathways and was looking disulfide-mediated aggregation by comparing reduced/non-reduced SDS page samples. I am using TCEP...

17 December 2020 2,724 3 View

How can I add details or change information to my article?

I missed to add all coauther to my publication. How can I add them??

10 December 2020 8,785 3 View

Do you know of a test dataset of research papers in computer science field?

Is there a freely available test dataset of research papers in computer science field? I need to evaluate my text retrieval system which uses a computer science domain ontology, so the dataset...

19 October 2020 5,412 3 View

Oxygen detection using Ru(bpy)?

I am trying to detect if my nanoparticles generate oxygen. Is there a way I can use Ru(bpy) and obtain an emission spectra? (Any other "quick and fast" method I can use to detect oxygen generation?)

26 August 2020 1,942 3 View

What are some in vitro experiments that can be performed to target hypoxia?

I want to show that my developed nanoparticle targets hypoxia but from doing research it seems like most hypoxic targeting related experiments are done in vivo. I'd like to be doing in-vitro...

16 August 2020 3,695 4 View

Can anyone help me understand this ROS protocol using CM-H2DCFDA dye?

Hi, this is my first time trying this ROS experiment and I am not a biologist or familiar at all with cell stuff. So I would appreciate if anyone can break down this procedure step by step; that I...

08 August 2020 6,857 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View