Please tell us process of protein aggregation.
Incubate at 56C for a few hours. Determine the amount of aggregation you are looking for/
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 179 3 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
Please note it
29 July 2024 4,920 3 View
https://www.cleanteqwater.com/resources/case-studies/gold-mine-australia/
29 July 2024 5,160 1 View
Cow dung manure is more powerful than artificial fertilizer.
19 July 2024 7,392 7 View
If the case of Irak is of December 2020 then i think I can suggest pl follow all the infection cause and death, already in our researchgate.
19 July 2024 3,356 2 View
Is this organic cow dung manure now important than artificial fertilizer.if good then why ?
19 July 2024 9,050 2 View
I have come to know one actor told publicly and media that was unable to attend marriage ceremony due to i was admitted in hospital of Mumbai.now negative so I am appeared again in front of...
17 July 2024 2,963 2 View
A group of students prefer frogs for dissection.
16 July 2024 4,847 1 View
If taken mice what benefits like humans will be available and human efficacy and mice?
16 July 2024 2,556 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,987 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,891 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,005 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,737 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,352 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...
21 July 2024 5,732 3 View